肠易激综合征幼鼠模型的建立及初步探讨  

Establishment on Infant Rats Model of Irritable Bowel Syndrome and for its Primary Exploration

作　　者：朱雪萍[1] 陈卫昌[1] 许诤[1] 吴旭东[1] 朱树养[1] 朱晓黎[1] 

机构地区：[1]苏州大学附属第一人民医院消化科,苏州215006

出　　处：《实验动物与比较医学》2007年第3期172-175,共4页Laboratory Animal and Comparative Medicine

摘　　要：目的建立幼鼠肠易激综合征(IBS)动物模型并评估其内脏敏感性。方法应用16只新生SD大鼠,随机分为模型组和正常对照组,模型组进行母子分离、改良机械束缚、结肠芥子油刺激等综合因素干预幼鼠,干预结束后休息2周,可建立IBS的动物模型;正常组不作干预。于生后30d用自制的扩张器对幼鼠进行直肠扩张,评估不同压力下腹部收缩反射(AWR)阈值,测定幼鼠腹壁肌电活动;检测结束后行结肠黏膜病理检测。应用Bristol分型观察各组粪便性状法进行评分,自干预开始收集粪便称湿重和干重。结果直肠扩张时,在不同压力下模型组腹部收缩反射(AwR)评分较对照组阈值显著降低(均P<0.01);不同扩张压力下,腹壁肌电活动随压力增加明显增强(P<0.01)。结肠病理检查两组均未见明显异常。模型组粪便多为软的团块或泥浆样,Bristol分型评分为5.3±0.8;而对照组为柔软的香肠状或团块状,Bristol分型评分为3.6±0.5,两组Bristol分型评分比较有显著性差异。模型组粪便湿重、干重分别为10.8±1,2g、3.2±0.4g;而对照组粪便湿、干重为5.4±0.3g、3.6±0.7g,两组粪便湿重存在显著差异(P<0.05)。结论用母子分离、改良机械束缚、结肠芥子油刺激等综合因素干预幼鼠可建立儿童IBS的动物模型,其机制可能为内脏敏感性增高。Objective To establish an infant rat model of irritable bowel syndrome with ＂integrative methods＂, and test its visceral sensitivity by two different ways. Methods Sixteen SD neonatal rats were divided into model and control group randomly. The model was established by separated from mother rat, binded up mildly and intrarectal administration of mustard oil and so on; the control group didn＇t intervene. After the intervention, let the rats have a rest for 2 weeks. The threshold of abdominal withdrawal reflex （AWR） was evaluated during rectal distension by self-trade dilator in different tensions, and the changes of rectal sensitivity were identified by the abdominal electrical activity measured at postnatal 30 days. At last pathological changes of the rectal mucosa was measured. At the beginning of intervention, the two groups＇ feces were scored according to the Bristol pattern, collected the feces and measured their wet and dried weights. Results The model group showed a significant decrease （P〈0.01） in the volume threshold of abdominal withdral reflex during rectal distension; there was a significant increase of abdominal electrical activity responding to different distension pressure compared with control rats （P〈0.01）. The colon＇s pathological changes had no significant difference in the two groups. The feces of model group were mostly soft mass or muddy, Bristol scores was 5.3 ± 0.8; which of control group were delicately sausage or mass, Bristol scores was 3.6 ± 0.5, the difference was significant in the two groups. The wet and dried weights of the model＇s feces were 10.8 ± 1.2 g, 3.2 ± 0.4 g respectively, and the control＇s were 5.4 ± 0.3 g, 3.6 ±0.7 g respectively, there was significant difference in the two groups＇wet weights（P〈0.05）. Conclusions The ＂integrative＂ methods （concluded to separation from mother, mechanism fetter and chronic rectal irritation with mustard oil） to the infant rats may establish the children＇s irritable bowel syndrome model,

关 键 词：肠易激综合征 内脏高敏感性 直肠扩张 肌电活动 

分 类 号：R-332[医药卫生] R574