制备结合凝血酶原复合物超声造影剂的初步实验研究  

Preparation of prothrombin conjugated ultrasound contrast agent: a preliminary experimental study

作　　者：杨莉[1,2] 刘政[1] 左松[1] 谭开彬[1] 高云华[1] 付赤学[1] 李秋颖[1] 

机构地区：[1]第三军医大学新桥医院超声科 [2]解放军第161医院特诊科,湖北武汉430010

出　　处：《中国医学影像技术》2007年第8期1111-1114,共4页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金项目(30471654)。

摘　　要：目的以超声空化栓塞肿瘤微循环为目的,制备一种结合凝血酶原复合物(PCC)的超声造影剂,评价其理化性质。方法采用机械振荡整合法和表面吸附法分别制备结合PCC的负电荷脂膜超声造影剂(浮选法洗涤)。观察并检测洗涤前后微泡大小、形态、浓度、荧光亮度,微泡与PCC的结合率及Ⅸ因子的活性。结果结合PCC微泡的浓度、形态与普通微泡比较无明显差异,但易静置分层。微泡与PCC的结合率及Ⅸ因子活性:两种制备方法间比较无明显差异(P>0.05),洗涤前与相同浓度的PCC比较Ⅸ因子活性无明显差异(P>0.05),但洗涤后结合率均值由洗涤前的96%左右降为82%左右(P<0.05),活性由90%左右降为19%左右(P<0.01)。结论机械振荡整合法与表面吸附法均可制备结合PCC的超声造影剂,微泡与PCC结合率较高,洗涤前能保持Ⅸ因子较高的活性,两种方法间比较无明显差异。　Objective To prepare prothrombin complex concentrate （PCC） conjugated anionic lipid microbubbles （MBs） and assess the physiochemical properties of the MBs, which is needed in further MBs-mediated ultrasound （US） therapy. Methods The anionic lipid MBs combined with PCC through two methods. One was by mechanical vibration, the other one by surface bind. Half of all resulted suspensions were used for repeated washing, using flotation method. The size, shape, bubble concentration, fluorescent intensity, the binding rate of microbubbles with FITC-PCC and the activities of factor Ⅸ in the suspension were measured and analyzed. Results The suspensions of PCC-conjugated anionic microbubbles appeared to delaminate quicker than the control group. The binding rates of MB and FITC-PCC, the activity of factor Ⅸ in two different preparations had no significant differences （P〉0.05）. There were no significant activity differences found of factor Ⅸ between the same concentration of PCC solution and the MB before washing （P〉0.05）. The washing did reduce the binding rates of fluorescence to MB from 96% to 82% （P〈0.05）. The activity of factor Ⅸ in PCC dropped from 90% to 19% after three repeated washing （P〈0.01）. Conclusion Prothrombin complex concentrate can be conjugated with lipid microbubbles through mechanical vibration or surface bind during preparation procedure. The conjugation of MB and PCC were firmly high, and can maintain the activities of factor Ⅸ. No significant differences were found in the two different preparations.

关 键 词：机械振荡 表面吸附 凝血酶原复合物 超声检查 造影剂 

分 类 号：R-33[医药卫生] R445.1