三磷酸腺苷结合转运蛋白G超家族成员2基因在胃癌组织中的表达及意义  

Expression of ATP-binding cassette transporter ABCG2 in gastric carcinoma and its significance

作　　者：王宁[1] 陈凛[1] 卫勃[1] 赵向阳[1] 鲁文静[2] 沈丽[2] 

机构地区：[1]解放军总医院普通外科,北京100853 [2]北京大学干细胞研究中心

出　　处：《中华胃肠外科杂志》2007年第5期450-453,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金资助项目（30570522）

摘　　要：目的探讨三磷酸腺苷结合转运蛋白G超家族成员2（ABCG2）在胃癌组织中的表达及意义。方法采用免疫组织化学（免疫组化）方法（IHC染色）检测ABCG2在45例手术切除胃癌组织和30例正常胃黏膜中的表达情况：应用RT—PCR和实时定量PCR检测30例胃癌患者的癌组织及胃切缘正常胃黏膜ABCG2的mRNA表达。结果ABCG2在胃癌原发灶组织中有较高程度的表达．其阳性率为62．2％；而30例正常胃黏膜中的阳性表达率为6．7％；两者比较P〈0.05。ABCG2在低分化腺癌中的表达高于中-高分化腺癌（P〈0.05）；胃癌组织中ABCG2mRNA水平的表达明显高于胃正常黏膜（P〈0.05）．与免疫组化结果一致。结论ABCG2在胃癌组织中过度表达．其可能在某种程度上参与了胃癌的发生和发展，可以作为胃癌肿瘤干细胞研究的重要分子。Objective To investigate the expression of ATP-binding cassette transporter （ABCG2） in gastric carcinoma and its clinical significance. Methods Expression of ABCG2 protein was examined by immunohistochemical technique in specimens from 45 gastric carcinoma tissues and 30 surrounding normal tissues. The expression of ABCG2 mRNA was measured by RT-PCR and realtime quantitative PCR in specimens from 30 gastric carcinoma tissues and 30 surrounding normal gastric mucosa respectively. Results ABCG2 protein expression was observed in 28 of 45 （62.2%） cases by immunohistochemical analysis, while in 2 of 30（6.7%） normal tissues（P〈0.05）. In ABCG2- positive tumors, adjacent non-neoplastic tissue was similarly analyzed and revealed that ABCG2 was up-regulated in gastric carcinoma. The positive rates of ABCG2 expression in poorly differentiated and/or undifferentiated carcinoma were significantly higher than those in well and/or moderatelydifferentiated carcinoma （P〈0.05）. The mRNA expression of ABCG2 was significantly higher in gastric carcinoma tissue than that in normal gastric mucosa （P〈0.05）. Conclnsions ABCG2 expression is upregulated in gastric carcinoma. ABCG2 may be an important factor in the research of gastric cancer stem cell.

关 键 词：胃肿瘤 三磷酸腺苷结合转运蛋白G超家族成员2 多药耐药 肿瘤干细胞 

分 类 号：R735[医药卫生—肿瘤]