阿托伐他汀对移植动脉慢性排斥反应的抑制作用及机制  

Depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats

作　　者：郭兴军[1] 宋自芳[1] 郑启昌[1] 张磊[1] 左克强[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院肝胆外科,湖北武汉430022

出　　处：《中国病理生理杂志》2007年第9期1676-1678,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30271242)

摘　　要：目的:研究阿托伐他汀(atorvastatin)对大鼠移植动脉慢性排斥的抑制作用及机制。方法:建立大鼠腹主动脉移植慢性排斥反应模型,分为3组:异系移植对照组、异系移植治疗组和同系移植组,60d后对移植动脉行病理组织学检测,观察移植动脉内膜增生程度;并行免疫组织化学染色(SP法),检测移植动脉增殖细胞核抗原(PCNA)和平滑肌肌动蛋白(α-SMA)的表达情况,行硝酸还原酶比色法测定移植动脉组织NO的含量。结果:异系移植治疗组血管内膜增厚程度(11.60%±2.40%)显著低于异系移植对照组(34.60%±6.40%,P<0.05),高于同系移植组(1.15%±0.65%,P<0.05);PCNA在异系移植治疗组(4.80%±0.80%)表达明显低于异系移植对照组(18.40%±1.80%,P<0.05),高于同系移植组(1.20%±0.40%,P<0.05)。结论:阿托伐他汀可抑制大鼠移植动脉慢性排斥反应,PCNA基因表达的下调可能是阿托伐他汀抑制移植动脉慢性排斥反应的机制之一。AIM： To investigate the depressant effect and mechanism of atorvastatin on the chronic rejection of aortic allograft in rats. METHODS ： The models of abdominal aorta transplantation were made with micro - surgery in rats. The recipients were divided into three groups： allograft control group, atorvastatin -treated group and isograft control group, Vascular intimal thickness in all of the groups was observed by histological examination, The expression of proliferation cell nuclear antigenl（PCNA） and α - smooth muscle actin（ α - SMA） were determined by immunohistochemistry, The content of nitric oxide was measured by nitrate reductase chromatometry, RESULTS ： The vascular intimal thickness in atorvastatin -treated group （11.60% ± 2. 40% ） was lower than that in allograft control group （34. 60% ± 6. 40%, P 〈 0. 05 ） and higher than that in i sograft control group （ 1, 15% ± 0. 65 %, P 〈 0. 05 ）, The expression level of PCNA was decreased in atorvastatin- treated group （4.80% ±0.80% ） than that in allograft control group （18.40% ± 1. 80%, P 〈 0. 05 ） and higher than that in isograft group （ 1. 20% ± 0. 40%, P 〈 0. 05 ）. CONCLUSION： The expression of PCNA in the transplanted aorta is suppressed by atorvastatin, which results in relief of chronic rejection of aortic allograft.

关 键 词：阿托伐他汀 移植 移植物排斥 增殖细胞核抗原 一氧化氮 

分 类 号：R363[医药卫生—病理学]