复方小柴胡汤对荷EAC鼠IL-2水平和CD4^+/CD8^+比值的影响  

Influence of Fu Fang Xiao Chai Hu Tang on level of interleukin-2 and value of CD4^+/CD8^+ in mice bearing Ehrlish ascites carcinoma

作　　者：方乐堃[1] 潘凌霄[1] 黎雅婷[1] 陈新桂[1] 崔丽萍[1] 林子洪[1] 郑芹[1] 杨惠玲[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室,广东广州510080

出　　处：《中国病理生理杂志》2007年第9期1720-1723,共4页Chinese Journal of Pathophysiology

基　　金：中山大学实验室开放基金资助项目(No.KF2005017);中山大学2006年学生暑期科研基金资助项目(No.SQ200626)

摘　　要：目的:探讨复方小柴胡汤(FFXCHT)对艾氏腹水癌(EAC)荷瘤小鼠脾细胞IL-2水平和血CD4+/CD8+比值的影响。方法:观察FFXCHT对EAC荷瘤小鼠的肿瘤重量、胸腺和脾系数的影响;用[3H]-TdR掺入法测定脾细胞IL-2的水平;用流式细胞仪测定血CD4+和CD8+T淋巴细胞含量,并计算CD4+/CD8+比值。结果:FFXCHT高、中和低浓度治疗组EAC荷瘤小鼠肿瘤重量均明显少于模型组(P<0.01)。模型组胸腺系数和脾系数高于空白对照组(P<0.05)。FFXCHT高、中和低浓度治疗组脾系数和胸腺系数均明显高于模型组(P<0.01)。与模型组相比,FFXCHT高、中和低浓度治疗均能明显提高荷瘤鼠脾细胞IL-2分泌水平(P<0.05)和血CD4+/CD8+T淋巴细胞比值(P<0.05),其中以中浓度治疗组最为明显。结论:FFXCHT能明显抑制EAC肿瘤的生长,提高胸腺和脾系数、脾细胞IL-2活性及血CD4+/CD8+比值,提示FFXCHT可能通过调整EAC荷瘤小鼠免疫功能而发挥其抗肿瘤作用。AIM： To investigate the effects of Fu Fang Xiao Chai Hu Tang （FFXCHT） on level of Interleukin - 2 and value of CD4^＋/CD8^＋ in mice bearing Ehrlish ascites carcinoma（ EAC ）. METHODS ： The effects of FFXCHT on the EAC were observed and index of thymus and spleen were observed. The method of [^3H] -TdR incorporation was used to measure the IL - 2 level, and the value of CD4^＋/CD8^＋ was assayed by ELITE calibur flow cytometry. RESULTS ： Compared with the model group, FFXCHT inhibited the growth of EAC （ P 〈 0. 01 ）. The index of thymus and spleen of the model group were higher than that in the control group （P 〈0. 05）. Compared with the model group, FFXCHT increased the index of thymus and spleen, the IL-2 level and the value of CD4^＋/CD8^＋ （P 〈0.05）, especially with the middle dose. CONCLUSION： The FFXCHT has antitumor effect. Increased IL - 2 level and the value of CD4^＋/CD8^＋ play a regulatory role in the immunologic function in mice bearing EAC.

关 键 词：复方小柴胡汤 荷EAC鼠 CD4-CD8比值 白细胞介素2 

分 类 号：R363[医药卫生—病理学]