大鼠骨髓间质干细胞诱导分化为心肌样细胞膜电流的研究  

Electrophysiological characteristics of the membrane current of cardiomyocytes-like cells derived from rat bone marrow mesenchymal stem cells

作　　者：农耀明[1] 宋治远[1] 尹戴佳佳 陈鹏慧[2] 

机构地区：[1]第三军医大学西南医院心内科 [2]第三军医大学神经生物教研室,重庆400038

出　　处：《中国病理生理杂志》2007年第9期1743-1746,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370585)

摘　　要：目的:研究大鼠骨髓间质干细胞(MSCs)诱导分化为心肌样细胞膜电流的状态。方法:按文献方法进行MSCs的诱导培养和鉴定。诱导后培养4周,用全细胞膜片钳技术,检测胞膜电流,并与未经诱导分化的MSCs进行比较。结果:未经诱导分化的MSCs不表达内向电流,只表达外向钾电流。MSCs经5-aza诱导后表达心肌特异性肌钙蛋白T,记录到2种膜内向电流,分别为钠电流(INa)和L-型钙电流(ICa),以及3种外向钾电流,包括瞬间外向钾电流(Ito),超快速激活延迟整流钾电流(Ikur)及缓慢激活延迟整流钾电流(Iks)。与未经诱导分化的MSCs相比,诱导后MSCs的钾电流以Ikur和Iks为主。结论:经5-aza诱导后,MSCs可分化成具有电压依赖性内向INa、ICa和外向Ito、Ikur、Iks的心肌样细胞。AIM： To study the electrophysiological characteristics of the membrane currents of cardiomyocytes - like cells derived from rat bone marrow mesenchymal stem cells （MSCs）. METHODS ： MSCs were induced, cultured and identified according to the reference. At the fourth week after treatment with 5 -azacytidine（5 -aza）, cardiomyocytes - like cells were detected for the membrane current with the whole - cell patch - clamp technique and compared with the undifferentiated MSCs. RESULTS： The undifferentiated MSCs only expressed potassium currents. MSCs were stained positive for troponin T after treatment with 5 - aza, and two kinds of inward currents and three kinds of outward currents were expressed. They respectively were the fast inward sodium current （ INa ）, the L - type calcium current （ICa）, the transient outward potassium current （ Ito）, the ultra - rapid delayed rectifier potassium current （ Ikur ） and the slow delayed rectifier potassium current （Iks）. Compared with the undifferentiated MSCs, the potassium currents of cardiomyocyteslike cells derived from MSCs were mainly made up of Ikur and Iks. CONCLUSION： After treatment with 5 -azacytidine, MSCs are differentiated into cardiomyocytes - like cells, which express the current of INa, ICa, Ito, Ikur and Iks.

关 键 词：骨髓间质干细胞 阿扎胞苷 大鼠 细胞分化 心肌样细胞 

分 类 号：R363[医药卫生—病理学]