不阻断升主动脉低温室颤左室引流技术对犬肺组织保护作用的实验研究  

Hypothermic ventricular fibrillation with left ventricular drainage under cardiopulmonary bypass on canine lung preservation without aortic-cross clamping

作　　者：李占清[1] 关晓海[1] 伊雪[1] 李立刚[1] 蒲国华[1] 陈晨[1] 张宇[1] 陈国生[1] 

机构地区：[1]华北煤炭医学院附属医院胸心外科,河北唐山063000

出　　处：《中国病理生理杂志》2007年第9期1772-1775,共4页Chinese Journal of Pathophysiology

摘　　要：目的:探讨体外循环不阻断升主动脉低温室颤左室引流技术对犬肺组织的影响。方法:14只健康成年杂种犬随机分为2组。常规建立体外循环,实验组:不阻断升主动脉,血流降温加心包腔内冰盐水诱导室颤;对照组:阻断升主动脉,经主动脉根部灌注冷晶体停跳液心脏停跳。用ELISA法、免疫组化法检测IL-8、NF-κB及ICMA-1的表达及光镜下观察犬肺组织形态学变化。结果:实验组血清IL-8浓度水平显著低于对照组(P<0.05)。实验组肺组织病理损伤较对照组轻;在CPB结束时,两组肺组织的NF-κB和ICAM-1的积分吸光度值(A值)显著增高(P<0.05),但对照组肺组织的NF-κB及ICAM-1的A值显著高于实验组(P<0.05)。结论:体外循环不阻断升主动脉低温室颤左室引流技术可减轻肺组织的损伤,对肺功能有一定的保护作用。AIM ： To evaluate the protective effect of hypothermic ventricular fibrillation without aortic - cross clamping under cardiopulmonary bypass（CPB） on canine lung. METHODS： Fourteen dogs were randomly divided into two groups. All dogs received a standardized anesthetic technique. A conditional CPB was performed in every instance. Ventricular fibrillation was induced by systemic hypothermia to 28 ℃ and pericardial cooling saline in the experimental group. A standard CPB was performed in control group. The concentration of IL - 8 in serum was measured by ELISA. The expressions of NF - κB and ICAM - 1 were determined by using immunohistochemical staining. RESULTS： Serum IL - 8 level in experimental group was significantly lower than that in control group （ P 〈 0. 05 ）. In experimental group, the pathological lesion of lung was improved, The results of histochemistry demonstrated that optical densities of NF - κB and ICAM - 1 in two groups were much higher than those of pre - CPB （ P 〈 0. 05 ）, the optical densities of NF - κB and ICAM - 1 in control group were much higher than those in experimental group （ P 〈 0. 05 ）. CONCLUSION ： Hypethermic ventricular fibrillation under cardiopulmonary bypass may be an effective method on reducing SIRS and protecting pulmonary function.

关 键 词：低温室颤法 肺损伤 NF-κB 胞间黏附分子1 白细胞介素8 体外循环 

分 类 号：R363[医药卫生—病理学]