Smo siRNA对LoVo细胞Smo基因表达及细胞增殖与凋亡的影响  

Effects of specific small interfering RNA on Smoothened expression and LoVo cell proliferation and apoptosis

作　　者：朱达坚[1] 方驰华[1] 戎祯祥[1] 刘胜军[1] 

机构地区：[1]南方医科大学珠江医院肝胆外科,广东广州510282

出　　处：《南方医科大学学报》2007年第9期1414-1417,共4页Journal of Southern Medical University

基　　金：佛山市卫生局医疗科研立项课题(2007373)~~

摘　　要：目的研究Smoothened(Smo)小干扰RNA(siRNA)对结直肠癌LoVo细胞Smo基因表达,以及LoVo细胞增殖与凋亡的影响。方法设计针对目标基因的siRNA,阳性脂质体介导转染LoVo细胞,半定量RT-PCR检测转染后LoVo细胞的Smo mRNA水平,MTT法和流式细胞术检测转染后LoVo细胞的增殖水平及细胞凋亡率。结果siRNA-1、siRNA-2均能抑制LoVo细胞Smo基因的表达,siRNA-1抑制作用最明显,达63.56%,siRNA-2抑制作用为46.54%,两组与隐性对照组相比,差异均具有显著性。siRNA-1转染LoVo细胞后,细胞增殖水平较隐性对照组显著降低(P<0.05);转染48h后,细胞凋亡率较隐性对照组显著升高(P<0.05)。结论Smo siRNA能有效地抑制结直肠癌LoVo细胞Smo基因表达,可以降低LoVo细胞的增殖水平,诱导细胞的凋亡。Objective To study the effects of specific small interfering RNA （siRNA） on Smoothened （Smo） gene expression and the proliferation and apoptosis of colorectal cancer LoVo cells. Methods Three different siRNAs （siRNA-1, siRNA-2, and siRNA-3, respectively） were transfected into LoVo cells via cationic liposome, and the changes of Smo mRNA level were determined using semi-quantitative RT-PCR 48 h after transfection. Flow cytometry and MTT assay were performed to assess the effect of the siRNAs on the proliferation and apoptosis of LoVo cells. Results Forty-eight hours after Smo siRNA-1 transfection, Smo mRNA expression in LoVo cells decreased by about 63.56%, a reduction significantly greater than that in cells transfected with the other two siRNAs. The cell proliferation decreased significantly after Smo siRNA-1 transfection in comparison with the control cells, and 48 h after transfection, significantly higher apoptosis rate was observed in Smo siRNA-1-transfected cells than in the control cells. Conclusion Specific siRNA can significantly decrease Smo mRNA expression and inhibit the proliferation while inducing apoptosis of LoVo cells.

关 键 词：结直肠肿瘤 Smoothened基因 RNA 小分子干扰 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]