动脉粥样硬化病变中p53抑癌基因结构突变的研究  

The studies of the mutations of p53 anti oncogene in Chinese adultsatherosclerosis

作　　者：王禹[1,2,3,4] 李天德[1,2,3,4] 谷志远[1,2,3,4] 刘建伟[1,2,3,4] 张笑明 刘国树[1,2,3,4] 杨庭树[1,2,3,4] 盖鲁粤[1,2,3,4] 郭宝玲[1,2,3,4] 

机构地区：[1]解放军总医院心内科 [2]解放军总医院分子生物学室 [3]解放军老研所分子生物室 [4]解放军总医院病理科

出　　处：《军医进修学院学报》1997年第1期13-13,共1页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金资

摘　　要：本文应用高灵敏、高特异的ＰＣＲ－ＳＳＣＰ方法，首次系统地研究了中国成人ＡＳ病变组织中ｐ５３ａｎｔｉ－ｏｎｃｏｇｅｎｅ的突变。结果发现：受检的８９例ＡＳ组织中的９例有ｐ５３基因的突变（有ＰＣＲ－ＳＳＣＰ的异常），其中６例经测序确定了ｐ５３基因突变的具体位点。结论：在人类的ＡＳ病变中确有ｐ５３ａｎｔｉ－ｏｎｃｏｇｅｎｅ的突变，此可能为ＡＳ形成过程中新的主要机制之一。Controlling by Chinese healthy adultsnormal artery cells and healthy adults normal blood lymphocytes(DNA), the mutations of p53 anti oncogene in 89 cases of Chinese adults atherosclerotic(AS) plaques were studied detailedly and systematically. The results showed that totally 9 cases(9/89) mutations of p53 gene were found by radiative PCR SSCP, a highest sensitive and highest specific method for the detection of DNA mutations, in which, 6 cases had been further verified by sequencing. In conclusion, from our studies, its clear that there are definite mutations of p53 anti oncogene in some Chinese adults AS plaque cells, and this phenomenon may be a new mechanism in the pathogenesis of human AS damages.

关 键 词：抑癌基因 动脉粥样硬化 突变 

分 类 号：R543.5[医药卫生—心血管疾病]