S100A9和NMP238在放疗敏感性不同宫颈鳞癌组织中表达的实验研究  

A study on expression of S100A9 and NMP238 in squamous carcinoma tissues of the cervix with different radiotherapy sensitivity

作　　者：曾亮[1] 朱红[2] 邓亚平[3] 裴海平[4] 袁君[2] 

机构地区：[1]湖南省肿瘤医院病理科 [2]中南大学湘雅医院肿瘤科,410008 [3]湖南省肿瘤医院细胞室,410006 [4]中南大学湘雅医院普外科,410008

出　　处：《临床肿瘤学杂志》2007年第9期646-650,共5页Chinese Clinical Oncology

基　　金：湖南省自然科学基金课题(06JJ4199);湖南省卫生厅课题(B2004-029)

摘　　要：目的:观察S100A9和NMP238在放疗敏感性不同宫颈鳞癌组织中的表达及临床病理学意义。方法:收集治疗前宫颈癌组织标本,病理诊断均为中分化鳞癌,置于-80℃超低温冰箱保存。在进行放疗后,根据WHO实体瘤放疗疗效判断标准,从中选择5例对放疗敏感的病例(高敏感组)和5例对放疗不敏感的病例(低敏感组),提取组织总蛋白,进行双向凝胶电泳和质谱分析鉴定差异表达蛋白,分别应用Western blot和免疫组织化学方法检测组织中蛋白的表达。结果:建立了分辨率高、重复性好的宫颈鳞癌放疗高敏感组和低敏感组的双向凝胶电泳图谱,差异表达蛋白经鉴定得到高敏感组中高表达的S100A9蛋白以及低表达的NMP238蛋白。Western blot检测结果显示S100A9在高敏感组中的表达强于低敏感组,NMP238在低敏感组中的表达强于高敏感组。免疫组化结果显示,S100A9在高敏感组中的阳性为表达率88.3%(53/60),高于低敏感组中的阳性表达率28.6%(10/35),二者差异有统计学意义(χ2=35.34,P<0.0001);NMP238在低敏感组中的阳性表达率为85.7%(30/35),高于高敏感组中的阳性表达率35%(21/60),二者差异有统计学意义(χ2=22.86,P<0.0001);S100A9在高敏感组中表达强度高于低敏感组,NMP238在低敏感组中表达强度高于高敏感组,差异亦均有统计学意义。结论:S100A9和NMP238的表达与宫颈鳞癌放疗敏感性有关。Objective：To investigate significance and expression of S100A9 and NMP238 in squamous carcinoma tissues of the cervix with different radiotherapy sensitivity. Methods：The fresh squamous carcinoma tissues of the cervix were collected from 10 untreated cervical carcinoma patients and preserved in the -80℃ refrigeratory. The tissues were classified into two groups： high sensitivity group（HS） and low sensitivity group（LS） according to their response to radiotherapy. Protein separating was performed by using two-dimensional gel electrophoresis （2-DE）, then the differentially expressed protein-spots were analyzed by mass spectrometry and data searching in the Mascot-database. Two differentially expression proteins were assayed by western blot and immunohistochemical method. Results：The gels were clear and successfully analyzed. It was found high expression of S100A9 in HS group, and high expression of nuclear matrix protein 238 were found in LS group. The higher expression of S100A9 in HS group and the higher expression of NMP238 in LS group were confirmed by western blot. Immunohistochemical result showed that S100A9 positivity rate （ 88.3% , 53/ 60） in HS group was significantly higher than that （ 28. 6%, 10/35 ） in LS group （χ^2 = 35.34, P 〈 0. 000 1 ） ; NMP238 positivity rate （85.7%, 30/35）） in LS group was significantly higher than that （85.7%, 30/35 ） in HS group （χ^ = 22. 86, P 〈 0. 000 1 ）. S100A9 expression was significantly stronger in HS group than in LS group and NMP238 expression was significantly stronger in LS group than in HS group. Conclusion ：S100A9 and NMP238 expression are assocaited with radiotherapy sensitivity of cervical squamous carcinoma.

关 键 词：宫颈癌 放疗敏感性 蛋白质组学 免疫组织化学 免疫印迹 

分 类 号：R730.55[医药卫生—肿瘤] R737.33[医药卫生—临床医学]