乳腺癌患者血浆RASSF1A基因启动子甲基化的意义  

Clinical significance of promoter hypermethylation of RASSF1A gene in plasma of breast cancer patients

作　　者：刘晓玲[1] 汪涛[1] 李明众[1] 

机构地区：[1]西安交通大学医学院第一附属医院放疗科,陕西西安710061

出　　处：《第四军医大学学报》2007年第18期1680-1682,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨乳腺癌患者血浆、肿瘤组织中RASSF1A基因启动子甲基化与临床病理等因素的相关性.方法:采用甲基化特异性PCR方法,分别检测68例乳腺癌患者血浆、肿瘤组织及癌旁腺体组织,以及12例良性乳腺疾病患者的血浆和乳腺组织中RASSF1A基因启动子甲基化状况.结果:乳腺癌组织RASSF1A基因启动子甲基化频率47.1%(32/68),患者血浆中同样DNA变异检出率33.8%(23/68).血浆中DNA甲基化状况与乳腺癌组织的甲基化状况显著相关(r=0.906,P=0.001).检测血浆中RASSF1A基因启动子甲基化的敏感性71.8%,特异性97.2%,肿瘤组织及血浆中游离DNA甲基化异常与临床分期,病理类型,肿瘤大小,ER,PR和cerbB-2状况无相关性(P>0.05).乳腺癌组织中未检测到RASSF1A基因启动子甲基化的血浆中、乳腺良性疾病患者血浆中均未检测到该基因甲基化变异.结论:乳腺癌患者血浆中RASSF1A基因启动子甲基化与肿瘤组织中的变异频率相关.AIM： To investigate the relationship between promoter methylation of tumor suppressor gene RASSF1A in plasma and clinicopathological features of breast cancer. METHODS： Methylation specific PCR was used to measure the promoter methylation status in plasma, paired tumor tissue and paracancerous normal tissue of 68 patients with breast cancer and in plasma and mammary gland of 12 patients with benign breast diseases. RESULTS： The detection rate of RASSF1A gene promoter hypermethylation was 47.1% （32/68） in tumor tissue and 33.8% （23/ 68 ） in paired plasma. RASSF1A gene hypermethylation in plasma was significantly correlated to that in tumor tissue （ r = 0. 906, P =0. 001 ）. The sensitivity of RASSF1A gene hypermethylation detection in plasma was 71.8% and the specificity was 97.2%. The aberrant methylation of RASSF1A gene in tumor tissue and plasma had no correlation to patients＇ age, tumor stage, tumor size, histological type, ER, PR, and receptor status （ P 〉 0.05）. No aberrant methylation of RASSF1A gene was found in the plasma samples from the patients with benign breast diseases and from the ones without gene methylation in tumor tissue. CONCLUSION： The aberrant RASSF1A gene promoter methylation in plasma of breast cancer patients is significantly correlated to that in breast cancer tissue.

关 键 词：乳腺肿瘤 RASSF1A基因 甲基化 

分 类 号：R737.9[医药卫生—肿瘤]