沙生冰草醇溶蛋白遗传多样性研究  

Genetic Diversity of Prolamines in Agropyron desertorum(Fisch ex Link) Schult

作　　者：车永和[1] 杨欣明[2] 杨燕萍[1] 李秀全[2] 何蓓如[3] 李立会[2] 

机构地区：[1]河北科技师范学院农学系,河北秦皇岛066600 [2]中国农业科学院作物科学研究所,北京100081 [3]西北农林科技大学农学院,陕西杨凌712100

出　　处：《麦类作物学报》2007年第5期767-771,共5页Journal of Triticeae Crops

基　　金：科技部项目(No.2004BA525B03);河北省教育厅重点项目(ZH2006001)

摘　　要：为了解中国北方不同地区沙生冰草居群的遗传结构和差异,应用醇溶蛋白A-PAGE技术对采集自中国沙生冰草主要生态环境的18个沙生冰草居群进行了遗传多样性研究。结果表明,沙生冰草具有丰富的遗传多样性。A-PAGE总计检测到64条相对迁移率不同的电泳带纹,在α?β?γ?ω区分别有11?17?6和30条。每个沙生冰草居群具有独特的醇溶蛋白带型,在确定和划分沙生冰草居群上显示出足够的遗传多样性。对地区内和地区间的醇溶蛋白遗传多样性进行划分,其中61.0%的总体遗传多样性存在于地区间,地区内遗传多样性占39.0%。在Nei遗传距离0.45处,18个沙生冰草居群被分为4个类群。UPGMA聚类和PCA分析表明,采集地生态环境相似的沙生冰草居群遗传距离较近。在沙生冰草的保护和利用中需加强对其生态环境和更多居群的保护和利用研究。To investigate the genetic variation and structure of Agropyron desertorum （Fisch ex Link） Schult. populations collected from various geographic areas in northern China, Eighteen populations of A. desertorum were used for prolamines analysis by A-PAGE. A high level of genetic diversity was detected in these A. desertorum populations. In total, 64 electrophoretic bands were observed following A-PAGE, among which 11, 17, 6 and 30 allelic variants were separated into α-,β-,γ-, and ω- fractions of prolamine. Each A. desertorum population had unique electrophoretic pattern, which exhibited rich genetic diversity for identification and classification of populations in A. desertorum. The proportion of diversity among and within geographic regions from which the populations of A. desertorum originated indicated that 39.0% of the total variation was found within regions, and 61.0% among regions. According to the dentrogram based on the Nei＇ genetic distance matrix, the A. desertorum populations from different regions were distinctly classified. At Nei＇s distance of 0.45, the populations of A. desertorum were divided into four groups. The UPGMA phenogram and the principal coordinate analysis indicated that the populations from similar eco-geographical situ＇ations were clustered together. More attention should be paid to greater numbers of populations of different regions in regard to collection and in preservation of A. deserorum germplasm.

关 键 词：沙生冰草 醇溶蛋白 遗传多样性 

分 类 号：S512.1[农业科学—作物学] S331