NF-kB抑制剂PDTC对人胃癌SGC-7901细胞凋亡及Livin和Caspase-3表达的影响  

The effect of PDTC on apoptosis and expression of Livin and Caspase-3 on human gastric carcinoma cell SGC-7901

作　　者：朱华云[1] 刘凌翔[1] 葛红梅[1] 王同杉[1] 刘平[1] 

机构地区：[1]南京医科大学第一附属医院肿瘤科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第8期816-820,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省医学重点人才"135"工程资助项目(52-2001)

摘　　要：目的:观察核因子-kB(NF-kB)抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)对人胃癌SGC-7901细胞生长及凋亡的影响,研究其对凋亡抑制蛋白Livin和Caspase-3表达水平的调控,探讨PDTC对人胃癌SGC-7901细胞凋亡影响的机制。方法:不同浓度的PDTC作用于SGC-7901细胞不同时间后,MTT法测定其对SGC-7901细胞增殖的抑制率;流式细胞仪观察SGC-7901细胞的凋亡情况;real time PCR法和Western blot法检测SGC-7901细胞Livin和Caspase-3 mRNA和蛋白的表达情况。结果:PDTC作用不同时间后对SGC-7901细胞生长有抑制作用,并诱导SGC-7901细胞凋亡,呈剂量-时间依赖性;PDTC可降低Livin mRNA及蛋白的表达,增加Caspase-3 mRNA及蛋白的表达,且Livin的表达量与PDTC的作用呈剂量,时间负相关。结论:PDTC可诱导人胃癌细胞株SGC-7901凋亡,其机制可能与PDTC抑制NF-kB信号转导通路,下调Livin表达继而上调Caspase-3表达有关。Objective：To detect the effect of PDTC,the selective inhibitor of NF-κB,on the growth and apoptosis of human gastric carcinoma cell SGC-7901,to analyze the expression of Livin and Caspase-3 of cell SGC-7901treated by PDTC,and to explore the mechanism of PDTC in induction of tumor cell＇s apoptosis.Methods：After the treatment with PDTC on human gastric carcinoma cell SGC-7901,MTr assay was used to observe the growth inhibition of cell SGC-7901.The cell apoptosis was assessed by flow cytometry （FCM）.The mRNA and protein expressions of Livin and Caspase-3 were detected by real time PCR and Western blot assay, respectively.Results： When PDTC was given,growth inhibition and elevated apoptosis of SGC-7901 cells were detected,which was showed in a dose-and time-dependent manner.PDTC also down-regulated the level of mRNA and protein expression of Livin,and elevated the mRNA and protein expression of Capase-3.Conclusion：PDTC can induce apoptosis of human gastric carcinoma cell SGC-7901 cells,which may be performed by down-regulating of Livin and up-regulating of Caspase-3 expression.

关 键 词：吡咯烷二硫代氨基甲酸盐 胃癌 细胞凋亡 核因子-KB LIVIN Caspase-3 

分 类 号：R735.2[医药卫生—肿瘤]