乙肝疫苗接种者和隐性HBV感染者血清中和效应的初步研究  

Initial research of neutralization effect on HBV infection by the sera of hepatitis B vaccine inoculators and the recovered patients with hepatitis B virus infection

作　　者：何小敏[1] 邢益平[1] 陈仁芳[1] 林燕[1] 韩亚萍[1] 刘婷[1] 刘雁雁[1] 李军[1] 黄祖瑚[1] 

机构地区：[1]南京医科大学第一附属医院感染科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第8期835-838,851,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(30371276)

摘　　要：目的:探讨乙肝疫苗接种者和隐性感染康复者血清体外阻断HBV感染作用的差异。方法:雅培ELISA定量测定乙肝疫苗接种应答者和隐性HBV感染康复者血清乙肝表面抗体,再调整到相同含量,分别和HBV预孵育1 h后感染Hep G2细胞,24 h后巢式PCR检测Hep G2细胞中HBV-DNA,评估其阻断效应。结果:同等浓度下,隐性感染康复者和乙肝疫苗接种者血清对野生株HBV感染阻断作用无差异,但乙肝疫苗接种者血清对变异株无阻断效应,隐性感染康复者对变异株仍有阻断作用,其作用明显弱于野生株。结论:隐性感染康复者血清对野生株和变异株HBV均有阻断作用,而乙肝疫苗接种者血清对变异株无阻断效应,提示新型乙肝疫苗应包含更多抗原成分如前S1/S2抗原,以对HBV变异株产生中和效应,应用多种成分的抗乙肝免疫球蛋白阻断肝移植后变异株HBV再感染十分重要。Objective：To investigate the difference between the sera of the hepatitis B vaccine inoculators and the recovered patients with acute infection in blocking hepatitis B virus infection in vitro.Methods：Titres of anti-HBs were tested by ELISA using Abbott kit in the sera of the vaccinates and the recovered patients with acute hepatitis B virus infection.Hep G2 cells were infected by wild type or＂a＂determinant mutant of HBV with or without pre-incubation for 1 h by the sera of the vaccinates（group 1）and the recovered patients with acute hepatitis B virus infection（group 2）.24 h later HBV DNA in Hep G2 cells were detected by PCR.The neutralization effect was determined based on the PCR results.Results：There is no significant difference between group 1 and group 2 in blocking wild type of hepatitis B virus infection to Hep G2 at the same concentration in vitro.The sera of group 2 can block the＂a＂determinant mutant strain HBV infection to Hep G2 cells,but the neutralization effect was weaker than that on wild type of HBV infection.The sera of group 2 have no neutralization effect on the＂a＂determinant mutant strain HBV infection.Conclusion：The sera of recovered patients with HBV acute infection can block both wild strain and＂a＂determinant mutant strain of HBV infection,while the sera of hepatitis B vaccine inoculators have no neutralizing effect on＂a＂determinant mutant strain.It suggested that the new hepatitis B vaccine should include more antigen component such as preS1/S2 to develop neutralization effect on＂a＂determinant mutant strain HBV,and more component anti-HBV antibodies was very important to protect HBV infected patients from mutant HBV reinfection after liver transplantation.

关 键 词：中和效应 HEP G2细胞 乙肝病毒表面抗原 前S1/S2抗原 乙型肝炎病毒 “a”决定簇变异 

分 类 号：R743.31[医药卫生—神经病学与精神病学] Q74[医药卫生—临床医学]