海洋真菌Trichoderma reesei的次级代谢产物的分离与鉴定  

Secondary metabolism product of fungus Trichoderma reesei

作　　者：孙奕[1] 吕阿丽[1] 田黎[2] 魏岚[3] 裴月湖[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]青岛科技大学生物制药系,山东青岛266042 [3]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第9期546-548,559,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家海洋863计划资助课题(GrantNO.2001AA624020)

摘　　要：目的系统研究海洋真菌Trichoderma reesei的次级代谢产物。方法利用硅胶柱色谱、高效液相色谱等手段进行分离,根据理化性质及波谱数据对所得结构进行了鉴定。结果从海洋真菌Trichoderma reesei发酵液的乙酸乙酯提取物中共分离得到6个成分,分别鉴定为cyclonerodiol(1)、8,9-dihydro-dihydroxymegastigmatrienone(2)、harzialactone A(3)、3,6-二苄基哌嗪-2,5-二酮(4)、3-异丁基-8-羟基吡咯并哌嗪-2,5-二酮(5)、3-苄基-8-羟基吡咯并哌嗪-2,5-二酮(6)。结论所分得的化合物均为从海洋真菌Trichoderma reesei的次级代谢产物中首次分离得到。Objective To study the secondary metabolism product of marine fungus Trichderma reesei sys- tematically. Methods The compounds were isolated by means of silica gel column and HPLC chromatography. Their structures were determined on the basis of physicochemical evidences and spectral methods. Results Six compounds were isolated from the ethyl acetate extracts of the fermentation of marine fungus Trichoderma reesei, which were identified as cyclonerodiol （1）, 8, 9-dihydroxy-megastigmatrienone （2）, harzialactone A （ 3 ）, 3, 6-dibenzylpiperazine-2, 5-dione （ 4 ）, 3-isobutyl-8-hydroxylpyrrolopiperazine-2, 5- dione （5）, 3-benzyl-8-hydroxyl-pyrrolopiperazine-2, 5-dione （6）. Conclusions Compounds 1-6 are isolated from marine fungus Trichderma reesei for the first time.

关 键 词：海洋真菌 瑞氏木霉 次级代谢产物 化学成分 

分 类 号：R283[医药卫生—中药学]