睡眠剥夺应激对幼鼠海马和视上核中谷氨酸受体及转运体表达的影响  

Expressions of NMDAR2 and GLAST in supraoptic nucleus and hippocampal gyrus under sleep deprivation in young rats

作　　者：王胜智[1] 王景杰[2] 马庆久[1] 何显力[1] 南菁[1] 秦云霞[1] 邱建勇[3] 

机构地区：[1]第四军医大学唐都医院普通外科,陕西西安710038 [2]第四军医大学唐都医院消化内科,陕西西安710038 [3]第四军医大学解放军神经科学研究所,陕西西安710032

出　　处：《医学研究生学报》2007年第9期927-930,共4页Journal of Medical Postgraduates

基　　金：陕西省科技攻关基金资助项目[批准号:2006K10-G(1)]

摘　　要：目的:观察睡眠剥夺对大鼠幼鼠视上核和海马组织谷氨酸受体NMDAR2及其转运体GLAST的影响,为防治因睡眠剥夺引起中枢功能的损害以及可能对学习、记忆产生的影响提供理论依据。方法:采用免疫组织化学的方法,观察与应激反应相关的视上核以及和学习、记忆密切相关的NMDAR2和GLAST表达的变化情况。通过灰度分析和荧光半定量分析的方法对实验结果进行处理。结果:视上核和海马均显示,在睡眠剥夺第3 d,NMDAR2和GLAST开始增加;睡眠剥夺第5 d,增加更为明显;睡眠剥夺的第7 d,增加的程度有所降低;睡眠剥夺第14 d,视上核NMDAR2和GLAST表达程度与正常对照组比较无明显差异。结论:睡眠剥夺对幼鼠视上核和海马的NMDAR2和GLAST的表达程度在一定的时间内有较为明显的影响。7 d后随着睡眠剥夺时间的延长,这种影响趋于消失,这可能与中枢神经系统自身调节和自我保护有关。Objective： To observe the expressions of NMDAR2 and GLAST in the hippocampal gyrus and supraoptic nucleus （SON） under sleep dep pressions of NMDAR2 and GLAST in SON and rivation in young rats. Methods： The changes in exhippocampal gyrus under sleep deprivation in younger rats were observed by immunohistochemistry method. Results：The expressions of NMDAR2 GLAST in SON and the hippocampal gyrus were significantly increased on the 3rd day of sleep dep and rivation, even more sigificantly on the 5th day, decreased gradually on 7th day, and became similar to those of control group on the 14th day. Conclusion： Sleep deprivation can affect the expressions of NMDAR2 and GLAST in the hippocampal gyrus and supraoptic nucleus, but the effect diminisshes gradually with prolonged time, which may be associated with self-regulation and self-protection of the central nervous system.

关 键 词：睡眠剥夺 海马回 视上核 NMDAR2 GLAST 

分 类 号：R338.26[医药卫生—人体生理学]