胶束电动毛细管色谱法和荧光偏振免疫法测定癫痫患者血浆中苯妥英浓度的比较  

Comparison of micellar electrokinetic capillary chromatography and fluorescence polarization immunoassay for determination of plasma phenytoin in epileptic patients

作　　者：芮建中[1] 赵瑜[1] 仇向华[1] 闵佩青[1] 朱志远[1] 李金恒[1] 

机构地区：[1]南京军区南京总医院药理科

出　　处：《医学研究生学报》2007年第9期950-952,956,共4页Journal of Medical Postgraduates

基　　金：江苏省卫生厅科研项目奥赛康临床药学研究基金资助项目(批准号:P200502);南京军区南京总医院科研基金资助项目(批准号:2004088)

摘　　要：目的:比较治疗药物监测中胶束电动毛细管色谱法(MECC)和荧光偏正免疫法(FPIA)测定血浆中苯妥英的浓度。方法:收集该院31例服用苯妥英的癫痫患者稳态浓度的血浆样品,分别以MECC和FPIA进行测定,用回归法考察两种方法测定结果的相关性。结果:两种方法无显著差异,具有良好的相关性(P>0.05)。回归分析的线性方程YFPIA=1.044×XMECC,相关性分析r=0.967。结论:MECC法可在无TDx仪的情况下替代FPIA,而且成本较低,快速简便。适用于苯妥英单用,以及苯妥英与常用抗癫痫药物(卡马西平、扑米酮、丙戊酸、苯巴比妥、氯硝西泮)合用的临床监测。Objective ： To compare micellar electrokinetic capillary chromatography （MECC） and fluorescence polarization immunoassay （FPIA） for the determination of plasma phenytoin in epileptic patients during routine therapeutic drug monitoring. Methods： Thirty-one steady-state plasma samples were collected from the epileptic patients treated with phenytoin. Both MECC and FPIA were used to determine the concentration of phenytoin. The results were analyzed by regression method in order to obtain the correlation between the two methods. Results： There was no significant difference between the results of the two methods （ P 〉 0.05 ）. The coefficient of correlation was 0. 967. Conclusion ： MECC is economical, reliable and quick for therapeutic monitoring of phenytoin in single administration or concomitantly with other antiepileptic drugs, such as carbamazepine, primidone, valproic acid, phenobarbital and/or clonazepam.

关 键 词：苯妥英 胶束电动毛细管色谱法 荧光偏振免疫法 

分 类 号：R742.1[医药卫生—神经病学与精神病学]