五氯酚钠的遗传毒性效应研究  

Genetoxicity of Sodium Pentachlorophenate (Na-PCP)

作　　者：徐韵[1] 李兆利[1] 李梅[1] 陈海刚[1] 孔志明[1] 刘征涛[2] 

机构地区：[1]污染控制与资源化研究国家重点实验室,南京大学环境学院,南京210093 [2]国家环境保护化学品生态效应与风险评估重点实验室,中国环境科学研究院,北京100012

出　　处：《南京大学学报（自然科学版）》2007年第4期372-376,共5页Journal of Nanjing University（Natural Science）

基　　金：国家"973"项目(2003CB415003)

摘　　要：五氯酚钠属有机氯农药,在世界范围内广泛使用,为了研究其对生态安全特别是水环境安全的潜在危害,采用小鼠骨髓细胞微核试验、蚕豆根尖微核试验和斑马鱼胚胎发育试验,评价了五氯酚钠的毒性效应.微核试验结果表明:对于小鼠骨髓细胞,50mg/kg和100mg/kg剂量组均能不同程度增加其微核率,和阴性对照组相比存在显著性差异(P<0.05),并存在一定的剂量-效应关系;对于蚕豆根尖细胞,各个剂量组均能不同程度增加其微核率,最高剂量组5.0mg/L和阴性对照组相比存在显著性差异(P<0.01),并存在一定的剂量-效应关系.斑马鱼胚胎发育试验结果表明:25℃时,五氯酚钠对斑马鱼胚胎发育有明显的致畸作用,72hEC50为515.31μg/L,可以看出胚胎异常率随暴露时间的延长和暴露剂量的增加呈现出增大趋势.微核试验和斑马鱼胚胎发育试验表明五氯酚钠具有一定的致突变性.Sodium pentachlorophenate （Na-PCP） is a kind of organic-chlorine pesticides, which is widely used all over the world. In China, sodium pentachlorophenate is used in controlling the schistosomiasis and acquiring the obvious effects. Sodium pentachlorophenate has a good water-solubility, but it degrades slowly in nature. Sodium pentachlorophenate can cause pollution by long-term and unsuitable use. To protect the environmental safety and human health, it is very important to studly the translation in the environment and organisms of sodium pentachlorophenate, clarify the mechanism of its toxicity and find the potential genetoxicity. In recent years, research of sodium pentachlorophenate focuses on the toxicity on animals, especially on aquatic animals. Due to the absent research on the mutagenicity to plants and toxicity to fish early life stage, people can＇t completely estimate the toxicity of sodium pentachlorophenate in the ecosystem. In this study, the toxicity effect of sodium pentachlorophenate was evaluated by using micronucleus tests of mouse bone marrow cells, Vivia faba root tip cells, and zebrafish embryo test, in order to research the potential danger of sodium pentachlorophenate for the ecological security, especially for the water environment. The results of micronucleus tests showed that the 50 mg/kg and 100 mg/kg dosages could induce a significant increase in micronucleus frequency of mouse bone marrow cells （P〈0.05） as compared with control and had a certain dose-effect relationship; only 5.0 mg/L dosage could induce an extremely significant increase in micronucleus frequency of Vivia faba root tip cells （P〈0.01） and had a certain dose-effect relationship. The results of zebrafish embryo test showed that 72 hrEC50 of sodium pentachlorophenate was 515.31μg/L, with a significant teratogenic effect on the embryo. The percentage of embryo abnormity increased with the exposure time and dosage. Micronucleus tests of mouse bone marrow cells, Vivia faba root tip cells, and zebrafish

关 键 词：五氯酚钠 小鼠 蚕豆 微核试验 斑马鱼胚胎发育试验 

分 类 号：X172[环境科学与工程—环境科学]