N-亚硝酰胺暴露水平与人胃粘膜pan-myc p62蛋白等的过表达  

INCREASED EXPRESSION OF p62^(pan-myc) IN GASTRIC PYLORI MUCOSA BIOPSIES FROM SUBJECTS EXPOSED TO N-NITROSAMIDES

作　　者：邓大君[1] 昌云生[1] 李彤 潘凯枫[1] 谷连坤 李吉友[1] 游伟程[1] 

机构地区：[1]北京医科大学临床肿瘤学院,100034

出　　处：《北京医科大学学报》1997年第1期9-11,共3页Journal of Peking University(Health Sciences)

基　　金：国家"八五"科技攻关资助

摘　　要：目的：从分子流行病学水平探讨myc等基因在体亚硝酸胺（NAD）致胃癌过程中的作用。方法：进行胃液总NAD含量测定和胃窦部粘膜活检及免疫组化染色。结果：57．1％的胃液样品检出NAD，pan－mycp62过表达率在胃液NAD阳性者和阴性者中差异显著（P=0．0239）；pan－rasp21、c－erbB－2P185和突变型P53的表达无此差异。结论：myc基因可能在NAD致人胃癌过程的早期发挥作用。To elucidate the possible role of myc oncogene in human gastric carcinogenesis byN-nitrosamides Methods: Expression of pan-myc in gastric biopsies of pylori and concentration of totalN-nitrosamides in fasting gastric juice samples were studied simultaneously with immunochemohistological method and liquid mobile-photohydrolysis-pyrolysis energy analyzer. Results: Ofgastric juice samples, 57. 1 % were N-nitrosamide-detectable. Evaluation of p62pan-myc in mucosal epithelial cells Was found in 6 gastric biopsies. All the 6 p62pan-myc-positive biopsies were from patients whosegastric juices were N-nitrosamide-detectable, whereas no p62 pan-myc-positive biopsy was found inthose from 15 cases whose gastric juices were N-nitrosamide-undetectab1e (P= 0. 0239). Such relationwas not observed for p21pan-ras, p185c-erbB-2, and p53mutant p53. Conclusion: These results indicate firstlythat expression of myc oncogene correlated with the exposure to N-nitrosamides in human stomach positively and myc oncogene may play a role in the early stage of human gastric carcinogenesis by N-nitrosamides.

关 键 词：亚硝酰胺 胃粘膜 原癌基因蛋白 胃肿瘤 

分 类 号：R735.202[医药卫生—肿瘤]