放射治疗对小鼠肾癌细胞凋亡和Bcl-2与Bax蛋白表达的影响  

The expressions of apoptosis related genes Bcl-2,Bax protein and apoptosis of renal carcinoma cells after radiotherapy in mice

作　　者：王瑶[1] 黄建华[1] 吕海燕[1] 郭佳[1] 杨军[2] 马林[2] 

机构地区：[1]解放军总医院基础医学研究所免疫学研究室,北京100853 [2]解放军总医院基础医学研究所放射治疗科,北京100853

出　　处：《感染．炎症．修复》2007年第1期11-13,F0003,共4页Infection Inflammation Repair

基　　金：北京市自然科学基金资助项目(7042060)

摘　　要：目的:观察放射治疗对BALB/c小鼠肾癌的抑制作用及对Bcl-2和Bax蛋白表达的影响。方法:建立BALB/c小鼠的肾癌细胞移植瘤放射治疗模型;HE染色测定肿瘤细胞死亡面积;采用免疫印迹法(Western blot法)检测肿瘤组织的凋亡相关蛋白表达。结果:放疗组小鼠移植瘤生长速度较肿瘤对照组明显减慢。放疗组小鼠肿瘤组织中抗凋亡蛋白Bcl-2表达下调,促凋亡蛋白Bax表达上调。结论:经放射治疗后,小鼠肿瘤生长受到明显抑制,可能与放疗引发肿瘤细胞凋亡相关蛋白表达变化相关。Objective： To observe the inhibitory effect of radiotherapy on expressions of Bcl-2 and Bax protein of renal carcinoma in BALB/c mice. Methods： Renal carcinoma cells were implanted in BALB/c mice,and tumor volume and weight were measured . The expression of Bcl-2,Bax,and Bak protein in implanted renal tun,ors were assayed by Western blot. Results： Growth of tumor mass was markedly slower after radiotherapy than that in untreated mice. The expression level of Bax was remarkably increased, the expression of Bcl-2 pretein was significantly decreased after radiation. Conclusion： The growth of renal carcinoma is markedly retarded in mice after radiation,and it might be attributed to changes in apoptosis related genes.

关 键 词：放射治疗Bcl-2 Bax肾癌 

分 类 号：R57[医药卫生—消化系统]