银杏内酯B对地塞米松诱导人外周血淋巴细胞凋亡的影响  

Effects of Ginkgolide B on the apoptosis of human peripheral blood lymphocytes induced by dexamethasone

作　　者：于力方[1] 廖杰[1] 王珊[1] 李宁[1] 梅世昌[1] 

机构地区：[1]解放军总医院医学实验测试中心,北京100853

出　　处：《感染．炎症．修复》2007年第2期98-99,F0003,共3页Infection Inflammation Repair

摘　　要：目的:探讨银杏内酯B(GB)对人外周血淋巴细胞凋亡的影响。方法:用淋巴细胞分离液体外分离外周血淋巴细胞,利用荧光显微镜观察经4’,6-联脒-2-苯基吲哚(DAPI)染色的淋巴细胞,随机分为3组:正常对照组,地塞米松处理组和银杏内酯B保护组。记录各组凋亡细胞核形态学改变并计算凋亡百分率。结果:地塞米松(Dex)体外可诱导淋巴细胞发生凋亡。与地塞米松组相比,银杏内酯B组淋巴细胞凋亡率明显降低,差异有显著性(P<0·05)。结论:银杏内酯B对人外周血淋巴细胞凋亡具有保护作用。Objective： To observed the effect of Ginkgolide B （GB） on the apoptosis of human peripheral blood lymphocytes. Methodss Human peripheral blood lymphocytes were separated by Ficoll in vitro. Apoptotic morphological changes and percentage of apoptosis were assayed with 4＇,6-diamidino-2-phenylindole （DAPI） staining and were observed under fluorescent microscope. Results： Dexamethasone caused apoptosis of the lymphocytes in vitro. After treatment with GB,there was a significant decrease in apoptosis of lymphocytes （P〈0. 05）. Conclusion： GB might exert protective effects on human peripheral blood lymphocytes against apoptosis.

关 键 词：细胞凋亡 淋巴细胞 银杏内酯B 

分 类 号：R735[医药卫生—肿瘤]