全反式维甲酸对HL-60细胞中N-乙酰氨基葡萄糖转移酶II,IV活力的影响  

Regulatory Effect of All trans Retinoic Acid(ATRA) on the Activities of N acetylglucosaminyl Transferase(GnT)III,IV in HL 60 Cells

作　　者：刘爱华[1,2] 李溪冰[1,2] 顾建新[1,2] 陈惠黎[1,2] 

机构地区：[1]卫生部糖复合物重点实验室 [2]上海医科大学生化教研室

出　　处：《生物化学与生物物理学报》1997年第2期116-121,共6页

基　　金：国家自然科学基金

摘　　要：本文研究全反式视黄酸（ＡＴＲＡ）对ＨＬ－６０细胞中乙酰氨基葡萄糖转移酶ＩＩ和ＩＶ（ＧｎＴ－ＩＩ，ＩＶ）的调控作用，结果发现０．１μｍｏｌ／Ｌ的ＡＴＲＡ即可使ＧｎＴ－ＩＩ和ＩＶ的活力明显降低至５０％左右，但ＡＴＲＡ浓度增至１．０或１０μｍｏｌ／Ｌ时，酶活力不再显著下降。在未经ＡＴＲＡ处理的对照细胞中，培养不同时间两酶活力均有较大的变动，未经同步化和同步化的细胞分别在２４ｈ及至４８ｈ达到高峰。ＡＴＲＡ处理后，未经同步化细胞的酶活力高峰也在２４ｈ，但不论同步化与否，在２４ｈ之前均未见两酶活力的下降，２４ｈ后，酶活力逐步降低。对以上结果作了讨论。The regulatory effect of all trans retinoic acid (ATRA) on the activities of N acetylglucosaminyl transferase(GnT) III and IV in HL 60 cells were studied. It was found that the activities of GnT III and GnT IV were significantly decreased by 0.1 μM ATRA,but not further decreased with the increase in concentration of ATRA to 1.0 or 10 μM. The activities of both GnT III and GnT IV in the untreated control cells were variable during the incubation of cells, showing a peak level at 24 h and 48h in the un synchronized culture and the synchronized culture respectively. After the treatment with ATRA, the peak value was still at 24 h in un synchronized culture , and the activities of GnT III and GnT IV were gradually decreased only after 24 h of incubation both in un synchronized and synchronized culture of HL 60 cells. The above results were discussed.

关 键 词：全反式 维甲酸 白血病 乙酰氨基葡萄糖 转移酶 

分 类 号：R733.702[医药卫生—肿瘤]