猪胰岛素前体在酵母Kluyveromyces lactis中的分泌表达及其转化为人胰岛素  

Secretory Expression of Porcine Insulin Precursor in Kluyveromyces lactis and Its Conversion into Human Insulin

作　　者：冯佑民[1] 张保焰[1] 张友尚[1] HiroshiFukuhara 

机构地区：[1]中国科学院上海生物化学研究所分子生物学国家重点实验室

出　　处：《生物化学与生物物理学报》1997年第2期129-134,共6页

摘　　要：通过将包括猪胰岛素前体（ＰＩＰ）基因在内的表达框架克隆至质粒ｐＫＤ１衍生的两种载体上而在酵母Ｋｌｕｙｖｅｒｏｍｙｃｅｓｌａｃｔｉｓ中分泌表达猪胰岛素前体。根据放射免疫测定结果，猪胰岛素前体的表达水平为２０～３０ｍｇ／Ｌ。猪胰岛素前体经过转肽被转变为基因工程人胰岛素。分析结果表明，来自Ｋ．ｌａｃｔｉｓ的人胰岛素，其氨基酸组成、晶体形状和生物活力与天然胰岛素相同。Porcine insulin precursor (PIP) was cloned to vectors derived from plasmid pKD1 and expressed in Kluyveromyces lactis. The secretory expression level of PIP was 20 to 30 mg per liter of the culture medium.Human insulin obtained from PIP through tryptic transpeptidation was characterized. Its amino acid composition, crystalline shape and biological activity are identical with those of native insulin.

关 键 词：猪胰岛素前体 酵母 基因工程 胰岛素 

分 类 号：Q578[生物学—生物化学] Q785