小麦体细胞无性系Glu-1基因突变体的遗传分析  

Genetic Analysis of a Glu -1 Somaclonal Mutant in Wheat

作　　者：张怀刚[1,2] 陈集贤[1,2] 胡含 

机构地区：[1]中国科学院西北高原生物研究所 [2]中国科学院遗传研究所

出　　处：《遗传》1997年第1期23-25,共3页Hereditas（Beijing)

基　　金：植物细胞与染色体工程国家重点实验室和青海省科委资助

摘　　要：对Ｇｌｕ－１基因的Ｇｌｕ－Ａ１和Ｇｌｕ－Ｂ１位点都发生突变的１个小麦体细胞无性系后代的高分子量谷蛋白亚基组成进行的分析结果，进一步证实了体细胞无性系发生了基因突变，且发现同源染色体的等位基因易发生相同突变，突变体多是纯合基因型。认为这是体细胞无性系变异稳定快的遗传根源。Evidence for specific gene mutation has been obtained in our previous research by analysing high-molecular-weight glutenin subunits (HMW-GS) controlled by Glu-1 gene in wheat somaclones. On the basis of the result, the present study detected in the offspring of a HMW-GS somaclone the mutants at both Glu-A1 and Glu-B1 loci. The occurrence of gene mutation in wheat somaclones was further proved. The results showed that a allele on homologous chromosomes often mutated identically. Genotype of this kind mutation migh be pure. Thie is considered to be the genetic reason for quickly stabilizing of somaclonal variation.

关 键 词：小麦 体细胞无性系 基因突变 遗传分析 

分 类 号：S512.103.2[农业科学—作物学]