云南汉族人群D17S30位点扩增片段长度多态性  

A Stud on Amplified Fragment Length Polymorphism at Locus D17S30 in Han Nationality of Yunnan Province

作　　者：欧炯文[1,2] 邹浪萍[1,2] 李建京 申滨[1,2] 韩晓华 赵永和[1,2] 

机构地区：[1]昆明医学院法医学系 [2]云南省高级人民法院法医处

出　　处：《遗传》1997年第2期11-14,共4页Hereditas（Beijing)

基　　金：云南省应用基础研究基金

摘　　要：应用ＰＣＲ技术和小型聚丙烯酰胺凝胶电泳银染法，对云南汉族人群Ｄ１７Ｓ３０位点扩增片段长度多态性进行了分析。在被检的１０５名无关个体中，共检出１２个等位基因，４１种基因型。等位基因频率范围在０００４８～０２１９０之间，杂合度为８３８１％，ＤＰ值为０９６４７。观察的基因型分布符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ定律。A study on amplified fragment length polymorphism (Amp-FLP) at locus D17S30 in Han nationality of Yunnan was carried out by using PCR followed by a high-resolution PAGE technique and silver staining. In a sample of 105 unrelated individuals, a total 12 different alleles and 41 genotypes were detected. The heterozygosity was 83.81% and the probability of discrimination(DP) was 0.9647. The distribution of observed genotypes obeyed the Hardy-Weinberg equilibrium.

关 键 词：人类遗传学 D17S30位点 扩增片段长度 多态性 

分 类 号：Q987[生物学—遗传学]