实验性肝硬化大鼠肠上皮细胞间紧密连接蛋白occludin表达下降  

INTESTINAL EPITHELIAL TIGHT JUNCTION PROTEIN OCCLUDIN DECREASED IN RATS WITH CCL4-INDUCED LIVER CIRRHOSIS

作　　者：马力[1] 崔巍[1] 鲁学恒[1] 

机构地区：[1]中国医科大学盛京医院感染病研究室,沈阳110004

出　　处：《中国组织化学与细胞化学杂志》2007年第4期416-418,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的研究实验性肝硬化大鼠大肠上皮细胞间紧密连接蛋白occludin表达的变化。方法参照文献1,给大鼠反复腹腔注射CCl4制备化学性肝硬化大鼠动物模型。实验4周、8周分批处死动物,应用免疫组织化学及Western blot检测肝硬化进程中,大鼠大肠上皮细胞间紧密连接蛋白occludin的定位及表达的变化。结果occludin蛋白主要沿大鼠大肠粘膜上皮细胞膜的顶端呈线状分布,在肝硬化组大鼠,4周时occludin的阳性染色开始减少,8周时更为明显。Western blot结果与免疫组织化学结果相一致,4周时开始下降(0.51±0.07),8周时达到最低值(0.32±0.05),与对照组(0.83±0.09)相比差异显著(P<0.05)。结论在肝硬化进程中,大肠上皮细胞间紧密连接蛋白occludin表达下降。Objective To investigate the changes of intestinal epithelial tight junction protein occludin in rats with CCl4-induced liver cirrhosis. Methods Male SD rats were divided into 2 groups. In one group, 30 ml/L CC14 （200ml/kg） were intraperitoneally injected twice a week for 8 weeks to induce hepatocirrhosis. Intraperitioncal injection of NS （2ml/kg） were given for controls. At the end of week 4 and 8, rats were killed and liver and intestinal specimens were reserved. Immunohistochemistry and Western blot were used to detect the distribution and expression of intestinal epithelial tight junction protein occludin. Results Tight junction protein occludin was localized to the apical region of the lateral plasma membrane representing the region of Tjs in surface and crypt epithelial cells. In mucosal tissue from rats with hepatocirrhosis in week 4 and 8, occludin staining was globally downregulated. Results from Western blot demonstrated consistent and significant reduction of occludin expression in rats with hepatocirrhosisin week 4 （0. 51 ±0. 07 vs0. 83 ±0. 09） and week 8 （0. 32 ±0. 05 vs0. 83± 0. 09） （P 〈0. 05 ）. Conclusion The expression of tight junction protein occludin decreased in intestinal epithelial cells of CC14-induced liver cirrhosis of rats.

关 键 词：肝硬化 大鼠 紧密连接蛋白 

分 类 号：R575.2[医药卫生—消化系统]