扩散性抑制对脑缺血后海马迟发性神经元死亡的影响  

EFFECT OF SPREADING DEPRESSION ON DELAYED NEURONAL DEATH IN RAT HIPPOCAMPUS AFTER MIDDLE CEREBRAL ARTERY OCCLUSION

作　　者：杜一星[1] 徐沙贝[1] 喻志源[1] 陈晨[1] 张强[1] 王伟[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2007年第4期419-423,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助项目(30230140;30600187)

摘　　要：目的为了研究阻断大鼠局灶性脑缺血诱导的扩散性抑制对同侧海马迟发性神经元死亡的影响。方法颈内动脉插线法制备大鼠大脑中动脉缺血再灌注模型,采用电生理学方法记录扩散性抑制波,尼氏染色和TUNEL染色检测海马迟发性神经元死亡;观察阻断局灶性脑缺血再灌注诱导的扩散性抑制对海马迟发性神经元死亡的影响。结果不给予SD阻断剂,大脑中动脉缺血模型有39%的动物出现海马迟发性神经元死亡;用MK-801阻断扩散性抑制后仅10%的动物出现海马迟发性神经元死亡,机率明显减小。结论局灶性脑缺血引起的海马迟发性神经元死亡可能与扩散性抑制由缺血区不断向远隔部位播散有关。Objective To explore the effect of blocking spreading depression which was induced by focal cerebral ischemia on the delayed neuronal death in ipsilateral hippocampus. Methods A rat model of middle cerebral artery occlusion was made by the intraluminal occlusion technique, the spreading depression waves were recorded by electrophysiology, and the effect of blocking spreading depression induced by ischemia on delayed neuronal death in the hippocampus was observed by Nissl＇s staining and TUNEL labeling. Results With no inhibitor of spreading depression, delayed neuronal death was detected in 39% of the animals in hippocampal CA1 region 3 days af- ter middle cerebral artery occlusion. With the inhibitor of spreading depression, delayed neuronal death appeared in only 10% of the animals. Conclusion Spreading depression might be related to delayed neuronal death in hippocam- pus after middle cerebral artery occlusion.

关 键 词：扩散性抑制 海马 迟发性神经元死亡 

分 类 号：R743[医药卫生—神经病学与精神病学]