油酸诱导慢性胰腺炎大鼠肝脏细胞IR及IRS-1蛋白表达的改变  

Protein expression of insulin receptor,insulin receptor substrate 1 of hepatic cell in experimental chronic pancreatitis rat induced by oleic acid

作　　者：赵战朝[1] 邱轶伟[1] 梁泉[1] 袁咏[1] 薛承锐[1] 

机构地区：[1]天津医科大学总医院中西医结合外科,天津300052

出　　处：《天津医科大学学报》2007年第3期373-375,共3页Journal of Tianjin Medical University

摘　　要：目的:观察慢性胰腺炎(CP)模型大鼠肝脏胰岛素受体(IR),胰岛素受体底物-1(IRS-1)蛋白表达的改变,探讨慢性胰腺炎继发性糖代谢异常的可能机制。方法:将用油酸诱导的CP大鼠作为模型组,同法注入生理盐水的大鼠为对照组,Western blot法测定各组大鼠肝细胞IR,IRS-1蛋白表达的改变。结果:模型组IR,IRS-1蛋白的表达较对照组都有明显降低(P<0.05)。结论:胰岛素信号转导异常可能参与了CP继发糖代谢异常的机制。Objective： To observe the changes of protein expression of insulin receptor, insulin receptor substrate 1 of hepatic cell of the model rat of chronic pancreatitis （CP） , so as to discuss the mechanism of diabetes secondary to chronic pancreatitis. Methods： The experimental model of chronic pancreatitis was induced by retrograde injection of oleic acid into the pancreatic duct. Protein expression of IR, IRS-1 was observed by western blot. Results： The model rats showed remarkable depression of IR, IRS-1 protein expression. Conclusion： Abnormality of insulin signal transduction may play a important role in the mechanism of metabolic of glucose in CP.

关 键 词：慢性胰腺炎 蛋白印记 胰岛素受体 胰岛素信号转导 大鼠 

分 类 号：R576[医药卫生—消化系统]