Smac基因对γ射线照射诱导的宫颈癌HeLa细胞凋亡的影响  

EFFECT OF SMAC GENE ON APOPTOSIS OF HeLa CELLS INDUCED BY γ-RAYS

作　　者：赵宝锋[1] 田梅[1] 阮建磊[1] 苏旭[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《辐射防护》2007年第5期272-276,共5页Radiation Protection

基　　金：中国博士后基金资助(2005038364);兰州重离子加速器国家实验室基金资助

摘　　要：将Smac基因全长cDNA转染HeLa细胞,以探讨Smac基因过表达对γ射线诱导的宫颈癌HeLa细胞凋亡的影响及其可能机制。转染24小时后Western Blot结果表明,转染Smac基因全长cDNA的HeLa/Smac细胞其Smac基因表达量与转染pcDNA3.1空载体的HeLa/pcDNA3.1细胞Smac基因表达量相比上调,而相反Smac基因的底物Survivin基因表达量下调。γ射线照射后HeLa/Smac细胞与HeLa/pcDNA3.1细胞相比凋亡率显著升高,Caspase-3活性上调,但HeLa/Smac细胞与HeLa/pcDNA3.1细胞相比DNA损伤及修复无显著变化,对细胞周期也无明显影响。To explore the effect of Smac gene on apoptosis of HeLa cells induced by γ-ray and its possible mechanisms,the full length eDNA of Smac gene was transferred into HeLa cells. 24 h after transferring, the results of Western Blot indicated the expression of Smac was increased but the expression of Survivin decreased. After HeLa cells was irradiated by γ-rays, Smac gene transferred HeLa/Smac cells showed more cell apoptosis rates and the higher activity of Caspase-3 than vector transferred control HeLa/pcDNA3.1 cells. However, the damage and repair of DNA and the cell cycle don＇ t change significantly, comparing HeLa/Smac cells with HeLa/pcDNA3.1 cells.

关 键 词：SMAC基因 Γ射线 放疗 基因治疗 HELA细胞 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]