喉鳞状细胞癌中Skp2和p27蛋白的表达  

Skp2 and p27 protein expression and their significance in laryngeal squamous cell carcinoma

作　　者：郭剑锋[1] 陈福进[2] 管志伟[1] 陈艳峰[2] 李秋梨[2] 欧阳电[2] 

机构地区：[1]东莞市人民医院耳鼻咽喉科,广东东莞523018 [2]中山大学肿瘤防治中心头颈科,广东广州510060

出　　处：《中国耳鼻咽喉头颈外科》2007年第9期519-522,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨细胞S相激酶相关蛋白(S-phasekinase associated protein 2,Skp2)、p27蛋白与喉鳞状细胞癌(简称喉癌)各临床因素及预后的相关性。方法采用免疫组化SP法检测79例喉癌患者肿瘤组织的Skp2、p27表达。结果喉癌中Skp2高表达率(53.16%)显著高于正常喉组织(0%,P<0.05);喉癌Skp2蛋白低表达组的5年生存率(72.18%)显著高于高表达组(44.17%,P<0.01)。p27蛋白在喉癌和癌旁喉组织中的高表达率分别为30.38%和90%,差异具有显著性(P<0.05);喉癌p27蛋白高表达组的5年生存率(72.98%)显著高于低表达组(51.13%,P<0.01)。将Skp2和p27结合分析,Skp2高表达并p27低表达组的5年生存率最低,与另一组相比具有显著性差异(P=0.001)。结论Skp2蛋白通过降解靶蛋白p27可能在喉癌发生、发展中发挥重要作用。　OBJECTIVE To investigate the correlation of S-phase kinase associated protein 2 （Skp2） and p27 expression with the clinical factors and prognosis of patients with laryngeal squamou cell carcinoma （LSCC）. METHODS The expressio of Skp2 and p27 in 79 specimens of LSCC and 1 specimens of adjacent normal laryngeal mucos tissues were evaluated by SP immunohistochemist methods. RESULTS The overexpression rate Skp2 was significantly higher in LSCC（53.16 %） tha in adjacent normal laryngeal mucosa tissue （0 % （P〈 0.05）. There was a significantly higher 5-yea survival rate in reduced Skp2 expression grou （72.18 %） compared with that in Skp2 overexpressio group（44.17 %）（P〈 0.01）. The overexpressio rate of p27 was significantly lower in LSCC（30.38 % than in adjacent normal laryngeal mucosa tissue （90 % （P〈 0.05）. There was a significantly higher 5-yea survival rate in p27 overexpression group （72.98 % compared with that in reduced p27 expressio group （51.13 %） （P〈 0.01）. Skp2 expression wa negatively associated with p27 expression. Patie with both Skp2 overexpression and reduced p2 expression revealed the poorest 5-year surviv rate （42.29 %）, and the 5-year survival rate other patients was 70.05 %. There was a significa difference（P =0.001）. CONCLUSION Th expression of Skp2 protein may lead to decreas p27 level in LSCC, indicating that it may play a important role in the pathway of carcinogenesis an progression of LSCC.

关 键 词：喉肿瘤 癌 鳞状细胞 S期激酶相关蛋白质类 周期素依赖激酶抑制剂P27 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]