增殖细胞核抗原和p27在鼻内翻性乳头状瘤中的表达  

Expression and significance of PCNA and p27 in inverted papilloma of nasal cavities and paranasal sinuses

作　　者：胡廷保[1,2] 文卫平[1] 孙德重[3] 陈慧敏[1] 许庚[1] 

机构地区：[1]中山大学附属第一医院耳鼻咽喉科医院,中山大学耳鼻咽喉科研究所,广东广州510080 [2]广州市番禺区人民医院耳鼻咽喉科,山东临沂276000 [3]山东省临沂市人民医院耳鼻咽喉科

出　　处：《中国耳鼻咽喉头颈外科》2007年第9期543-546,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的检测鼻内翻性乳头状瘤(nasal inverted papilloma,NIP)组织中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)和p27蛋白表达,从细胞增殖状况和细胞周期调控方面探讨NIP发生、生长机制。方法应用免疫组织化学LSAB法检测47例NIP、49例鼻息肉、43例鼻鳞状细胞癌、11例正常鼻黏膜组织中PCNA和p27蛋白的表达。结果NIP组PCNA标记指数(labelling index,LI)高于鼻息肉组及正常鼻黏膜组(P<0.001),与鼻鳞状细胞癌组差异无统计学意义(P>0.05),NIP中初发组与复发组之间差异无统计学意义(P>0.05);p27蛋白在各实验组中均有部分表达,各组间差异无统计学意义(P>0.05)。结论PCNA指数可以反映NIP的增殖状况,但其与NIP的复发无明显关系;而p27在NIP发展过程中的作用尚需进一步探讨。　OBJECTIVE To investigate the expression of proliferating cell nuclear antigen （PCNA）and p27 protein in inverted papilloma （NIP）of nasal cavities and papransal sinuses and to explore the mechanism of the formation and growth of NIP from cell proliferating condition and cell cycle regulation. METHODS PCNA, p27 protein were stained with immunohistochemical technique （LSAB）. There were four groups of patients47 cases in the NIP group, 49 cases in the nasal polyps group, 43 cases in the nasal squamous carcinoma group and 11 cases in the normal control group. RESULTS The PCNA labeling index（LI）in NIP group was significantly higher than that in the groups of nasal polyps and normal nasal mucosa （P〈0.001）. But no significant difference was found between NIP and nasal squamous carcinoma group（P〉0.05）. At the same time, there was no difference between primary NIP and recurrent NIP group （P〉0.05）. p27 protein was expressed in all groups, but no difference was found among them （P〉0.05）. CONCLUSION The PCNA labeling index may reflect the proliferating condition of NIP, but does not have relationship with NIP recurrence. And the role of p27 in the development of NIP needs more investigation.

关 键 词：乳头状瘤 内翻 鼻 鼻窦 增殖细胞核抗原 周期素依赖激酶抑制剂P27 免疫组织化学 

分 类 号：R739.62[医药卫生—肿瘤]