沼泽红假单胞菌的选育及对酚降解性能探讨  

Selection of Phenol-degrading Mutants of Rhodopseudomonas palustris and Comparison of Their Phenol-degrading Capacities

作　　者：吴芳芳[1] 胡秀芳[2] 李璐[1] 张家琦[1] 蒋慕贤[1] 

机构地区：[1]南京信息工程大学环境科学与工程学院,江苏南京210044 [2]浙江理工大学生物工程研究所,浙江杭州310018

出　　处：《农业环境科学学报》2007年第5期1889-1893,共5页Journal of Agro-Environment Science

基　　金：南京信息工程大学科研基金Y510项目;863课题2006AA10Z428项目

摘　　要：分别用紫外线、N+离子束和硫酸二乙酯作为诱变剂,对沼泽红假单胞菌Rhodopseudomonaspalustris进行单因素诱变和复合诱变,筛选高效降酚菌株。结果表明,在单因素诱变中,N+离子注入后的突变株,生长速率加快,脱氢酶活性较对照菌提高14.89%,该提高率是紫外诱变株和化学诱变株的2倍。复合诱变菌株菌落颜色明显加深,其生长速率和脱氢酶活性均比单因素诱变菌大。在以苯酚作为唯一碳源的培养基上,单因素诱变菌株和复合诱变菌株对酚均有较好的降解能力,其中复合诱变菌株在含酚2000mg·L-1的培养基中降解培养72h,对酚的去除率达94%,对高浓度含酚工业废水的酚降解率达81.20%,降解效果均显著好于单因素诱变菌,说明N+离子注入诱变是筛选沼泽红假单胞菌更有效的方法,复合诱变使沼泽红假单胞菌获得了更多的优良性状,其突变株的环境适应能力比单独诱变的好。Phenol-degrading mutants of Rhodopseudomonas palustris were obtained with single and complex mutation treatments. Ultraviolet, N^＋ion and diethyester sulphate （DES） were used as mutagen inducing mutants of Rhodopseudomonas palustris, individually or multiply. The results indicated that the growth rate of the mutant induced by N^＋ ion increased compared to the initial strain, and that the activity ofdehydrogenase increased by 14.89%, two times that of the mutants induced by UV and DES. As for the mutants with complex-mutation, their colonies color deepened, and the growth rates along with dehydrogenase activities were obviously higher than that of both the initial strain and single mutant by UV, DES and N^＋ion. The degradation efficiencies of phenol by compound mutants were higher than that by single mutants in culture medium with phenol as the only carbon source. The phenol degradation rates by compound mutants were 94% and 81.20% in the medium with 2 000 mg·L^-1 phenol and in the wastewater containing phenol, respectively. N^＋ mutation was more efficient than ultraviolet and DES mutation in breeding phenol-degrading Rhodopseudomonas palustris. The mutants induced by complex-mutation had more physiological characters and higher adaptability than other mutants.

关 键 词：沼泽红假单胞菌 复合诱变 降解 含酚废水 

分 类 号：X172[环境科学与工程—环境科学]