维生素E琥珀酸酯对前列腺癌PC-3细胞株的生长抑制和诱导凋亡作用  

Vitamin E Succinate inhibits growth and induces apoptosis of prostate cancer cell line PC-3

作　　者：虞力航[1] 杨波[1] 王林辉[1] 孙颖浩[1] 

机构地区：[1]第二军医大学长海医院泌尿外科,上海200433

出　　处：《第二军医大学学报》2007年第9期956-959,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30400444).~~

摘　　要：目的:探讨维生素E琥珀酸酯(vitamin E succinate,VES)对于激素非依赖前列腺癌PC-3细胞株的生长抑制和诱导凋亡作用。方法:将VES溶解于无水乙醇,配制成VES溶液。取对数生长期的PC-3细胞,分别加入25、50、75、100、125 mg/L VES溶液,对照组加入1.25%浓度乙醇。培养24、48、72 h后,采用MTT法分析细胞的活力指数和抑制率,采用流式细胞术检测PC-3细胞的细胞凋亡率。结果:实验组的细胞活力明显低于对照组(P<0.05),且与VES的作用浓度和作用时间呈负相关,而对照组的细胞活力与时间呈正相关。实验组的凋亡率较对照组有明显的升高,且与VES的浓度和作用时间呈正相关。并且,经VES作用48 h后,75 mg/L的VES浓度可以对PC-3细胞起到一个最佳的诱导凋亡作用,造成80%以上的PC-3细胞凋亡。结论:VES具有抑制PC-3细胞增殖及促进其凋亡的作用。提示其对于前列腺癌的化学预防和杀伤作用具有临床应用前景。Objective： To study the inhibitory and apoptosis-inducing effect of vitamin E succinate （VES） on androgen-independent prostate cancer cell line PC-3 in vitro. Methods： VES was dissolved with ethanol to obtain VES solution. PC-3 cells of logarithmic growth phase were treated with various concentrations of VES solution （25,50,75,100,and 125mg/L）; cells in control group were treated with 1.25% ethanol. MTT method was used to measure the viability and inhibitory rate of cells in each group 24h,48h and 72h after VES treatment; flow cytometry was employed to determine the apoptosis rate of the PC-3 cells. Results： The viability of cells in the experimental groups was significantly lower than that in the control group （P〈0.05）. The viability of cells in the experimental groups was negatively correlated with the concentration and exposure period of VES solution; the viability of cells in the control group was positively correlated with the exposure period of VES solution. The apoptosis rate of cells in the experimental groups was much higher than that in the control group; the rate in the experimental group was positively correlated with the concentration and exposure period of VES solution. The optimal induction of apoptosis was achieved after 48 h exposure to 75 mg/L VES solution, with a apoptosis rate above 80%. Conclusion： VES can inhibit the proliferation of PC 3 cells and can induce apoptosis of them,which casts new lights on prevention and treatment of prostate cancer.

关 键 词：维生素E琥珀酸酯 前列腺肿瘤 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]