β-catenin在慢性粒细胞白血病中的表达及与bcr/abl的关系  

Expression of β-Catenin Gene in CML and Its Relationship with bcr/abl

作　　者：李增军 邱录贵 李新 麦玉洁 王国蓉 于珍 王亚非 李长虹 李茜 

机构地区：[1]中国医学科学院、北京协和医学院,血液学研究所,血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2007年第5期931-935,共5页Journal of Experimental Hematology

基　　金：天津市自然科学基金资助项目;编号06YFJMSC08500;人事部留学人员科技活动择优资助项目

摘　　要：本研究检测β-catenin在慢性粒细胞白血病(CML)各期中的表达情况,并与bcr/abl的表达变化相比较,为进一步探讨β-catenin在CML急性变中的意义提供依据。首先分离CML各期患者及正常供者骨髓单个核细胞(BMMNC),提取总RNA,逆转录为cDNA,用实时定量PCR的方法检测β-catenin的表达情况,同时检测部分标本bcr/abl的表达情况,分析二者在CML进展过程中的表达变化及两者的相关性。结果表明:β-catenin在CML急性变期BMMNC的表达明显高于慢性粒细胞白血病(CML)慢性期(p<0.001)、加速期(p=0.016)及正常人(p=0.004),而在后三者之间未见统计学差异。急性变期bcr/abl的表达明显高于慢性期(p=0.001)。β-catenin的表达量与bcr/abl表达水平有明显的相关性(r=0.620,p<0.001)。结论:CML急性变期β-catenin的表达明显升高,并与bcr/abl的表达量有相关性。β-catenin的表达增高可能与CML急性变有关。This study was aimed to quantitatively detect the expression level of β-catenin and bcr/abl in different phases of chronic myeloid leukemia （CML） and to analyze their potential relationship and significance in the progression of CML. First, the total RNA isolated from BMMNC of patients with CML and donors was reversely transcribed into cDNA. The real-time quantitative PCR method was used to analyze the expression level of β-catenin and bcr/abl. The expression level of β-catenin and bcr/abl in different phases of CML was compared and the correlation was analyzed between the two genes. The results showed that the β-catenin gene in BMMNC of blast crisis of CML patients was expressed significantly higher than that in chronic phase （p 〈 0. 001 ） and accelerated phase （p = 0.016 ）of CML patients and in normal donors（p =0.004）, The expression of bcr/abl in blast crisis of CML was statistically higher than that in chronic phase of CML（p =0.001 ）. The expression levels of β-catenin and bcr/abl were correlated with each other in CML patients（ r = 0. 620, p 〈 0. 001 ）. It is concluded that the β-catenin gene in blast crisis of CML patients express higher than that in chronic phase and accelerated phase of CML, and its expression level is correlated with the level of bcr/abl expression. The increased expression of β-catenin may be account partly for the blast crisis of CML.

关 键 词：慢性粒细胞白血病 慢性粒细胞白血病急性变期 BCR/ABL基因 Β-CATENIN 表达 

分 类 号：R733.7[医药卫生—肿瘤]