肾母细胞瘤中Stat3 HIF-1α及VEGF的表达及意义  

Expression of Stat3,HIF-1α and VEGF in Wilms′ tumor

作　　者：牛之彬[1] 王常林[1] 侯英[1] 杨屹[1] 高红[2] 

机构地区：[1]中国医科大学附属盛京医院小儿外科,辽宁沈阳110004 [2]中国医科大学附属盛京医院小儿先天畸形重点实验室,辽宁沈阳110004

出　　处：《中国当代儿科杂志》2007年第5期461-464,共4页Chinese Journal of Contemporary Pediatrics

摘　　要：目的研究血管内皮生长因子（vascular　endothelial　growth　factor，VEGF），信号传导与转录激活因子3（signal　transducer　and　activator　of　transcription3，Stat3）和低氧诱导因子HIF-1α（hypoxia-induciblefactor-1α，HIF-1α）在肾母细胞瘤（Wilms’tumor，WT）中的表达及其临床意义。方法应用免疫组化SABC法辅以计算机图像分析的方法，研究Stat3，HIF-1α与VEGF在52例WT组织，47例瘤旁组织及8例正常肾组织表达强度情况。结果VEGF，Stat3及HIF-1α在WT组织中表达强度较瘤旁组织及正常肾组织显著增强（P〈0．05），且瘤旁组织中VEGF表达强度高于正常肾组织。另外，临床分期Ⅲ～Ⅳ和预后不良病理类型的WT中Stat3及VEGF表达强度明显高于临床分期Ⅰ～Ⅱ的WT，预后不良病理类型及直径≥6cm的WT中HIF-1α表达强度较预后良好型及直径〈6cm的WT升高。结论VEGF，Stat3及HIF-1α表达与肾母细胞瘤的发展预后有关，参与了肿瘤血管的生成及肿瘤的增殖侵袭，Stat3可能对HIF-1和VEGF的表达起着重要的调控作用，对于靶向治疗肾母细胞瘤具有一定意义。Objective To study the expression of signal transducer and activator of transcription 3 （ Star3）, hypoxia- inducible factor-1α （HIF-1α） and vascular endothelial growth factor （VEGF） in Wilms＇ tumor and their roles in the development of Wilms＇ tumor. Methods The expression of Star3, HIF-1α and VEGF were detected by the immunohistoehemical staining in 52 specimens from Wilms＇ tumor tissues, 47 from adjacent kidney tissues and 8 from normal kidney tissues. The expression intensity was analyzed by computer image processing. Results The expression of Star3, HIF-1 and VEGF were significantly up-regulated in Wilms＇ tumor tissues compared to those in adjacent tissues and normal kidney tissues （ P 〈 0.05 ）. Star3 and VEGF proteins in Wilms＇ tumor tissues of stage Ⅲ-Ⅳ and high risk histopathology were significantly higher than those of stage Ⅰ - Ⅱ and low risk histopathology. The higher expression of HIF-1 in Wilms＇ tumor tissues was shown in tumors with high risk histopathology and tumor size ≥ 6 cm. Conclusions Increased expression of Stat3, HIF-1 and VEGF were found in Wilms＇ tumor tissues, and may be related to the development and angiogenesis of Wilms＇ tumor. Stat3 may regulate the expression of HIF-1 and VEGF, so it could be an effective target for inhibiting VEGF expression and angiogenesis of Wilms＇ tumor.

关 键 词：信号传导与转录激活因子3 低氧诱导因子1Α 血管内皮生长因子 肾母细胞瘤 血管生成 

分 类 号：R737.11[医药卫生—肿瘤]