膀胱癌多药耐药基因MDR-1与金属硫蛋白-3表达的关系  

Relationship Between Expressions of MT-3 and MDR-1 in Bladder Cancer

作　　者：朱斌[1] 杨罗艳[1] 赵晓昆[1] 

机构地区：[1]中南大学湘雅二医院泌尿外科,长沙410011

出　　处：《癌变．畸变．突变》2007年第5期412-415,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：背景与目的:探讨膀胱移行细胞癌中多药耐药基因(MDR-1)与金属硫蛋白-3(MT-3)的表达及其相互关系。材料与方法:采用RT-PCR对11例正常膀胱黏膜和23例膀胱移行细胞癌(BTCC)组织MT-3和MDR-1mRNA表达情况进行分析。结果:11例正常膀胱黏膜组织中MDR-1和MT-3mRNA表达阳性率分别为9.1%(1/11)和0%(0/11),23例膀胱癌组织中MDR-1和MT-3表达阳性率分别为56.5%(13/23)和78.3%(18/23);膀胱移行细胞癌组织与正常膀胱黏膜中MDR-1、MT-3表达阳性率相比,差异均有统计学意义(P<0.05),而MDR-1与MT-3的表达无明显相关关系(P>0.05)。结论:MDR-1可能为肿瘤组织分化程度的一个标志,MT-3的表达与膀胱移行细胞癌的早期诊断相关,可能成为一种新的肿瘤标志物。BACKGROUND ＆ AIM： To investigate the role of MT- 3 in transitional cell carcinomas of bladder and the possible relation with MDR-1. MATERIALS AND METHOD： Using RT-PCR technique, the expressions of MT-3 and MDR-1 mRNA in 11 specimens from normal bladder mucosa and 23 specimens from bladder transitional cell cancer （BTCC） were measured. RKSULTS： MT-3 and MDR-l-positive expression rates were found in 0 and 9.1% in normal bladder mucosa , respectively .But they were 78.3% and 56.5% in 23 BTCC specimens , respectively. The positive rate was significantly higher in BTCC specimens than in normal tissue （P 〈 0.05） . The expressions of MT-3 and MDR-1 were not related with the grade , stage and recurrence after postoperative intravesical chemotherapy. CONCLUSION： MT-3 mRNA could not be identified in normal bladder mucosa, but highly expressed in bladder neoplasm, MT-3 might be used as a tumor marker.

关 键 词：膀胱移行细胞癌 金属硫蛋白 多药耐药性 

分 类 号：R737.14[医药卫生—肿瘤]