Bio-oss/rhBMP-2复合体修复异种再植牙周围骨缺损的实验研究  

Study of a Bio-oss and rhBMP-2 composite repairing the alveolar bone defects around heterogenous implanted teeth

作　　者：陈志方[1] 叶茂昌[2] 丁晓红[1] 黄忠锁 

机构地区：[1]合肥市口腔医院口腔外科,230001 [2]安徽省立医院口腔医学中心

出　　处：《实用口腔医学杂志》2007年第5期644-647,共4页Journal of Practical Stomatology

基　　金：合肥市科委医药卫生类重点资助项目(编号:20051001)

摘　　要：目的:评价Bio-oss人工骨与重组人骨形成蛋白-2(rhBMP-2)复合体修复异种再植牙周围骨缺损的效果。方法:拔除3只普通狗下颌第3前磨牙,扩大牙槽窝,造成颊舌侧牙槽骨完全缺损,将临床拔除的锥形多生牙根、经Ca(OH)2根充并干燥植入后,在牙根周围缺损区植入Bio-oss/rhBMP-2复合体,术后2、3个月通过X线影像学、硬组织切片进行组织形态学定性分析。结果:2个月时,骨缺损区新骨逐渐开始替代Bio-oss并钙化,新骨与再植牙根面和基骨形成骨性结合;3个月时新生骨骨小梁的面积(B.Ar)增加,骨小梁周长(B.Pm)减小(P<0.05),骨小梁厚度增大,未完全降解的Bio-oss颗粒被逆根向转移。结论:Bio-oss/rhBMP-2复合体可有效促进再植牙周围骨缺损区骨组织再生,并促使牙根与牙槽骨形成根-骨结合。Objective： To evaluate the reliability and effects of a compound bone substitute materials Bio-oss artificial bone grafts with recombinant human bone morphogenic protein-2（rhBMP-2） filling the alveolar bone defects around heterogenous replanted teeth. Methods：3 dogs＇ bilateral mandible 3rd premolars（6 sites） were extracted. The defect sites without buccal and lingual alveolar bone were prepared for the heterogenous implanted teeth and the compound of Bio-oss and rhBMP-2 filled the alveolar bone defects around. The bone regeneration was observed by naked eye, radiograph, histology and histomorphometric analysis after 2, 3 months. Results： The new alveolar bone showed osteogenesis and calcification after 2 months, and intergrated with implanted teeth root under the light microscope and X ray. The new bone trabecular thickness（Tb. Th） augmented continuously. Differences of the bone area（ B. At） and bone perimeter（ B. Pm） were statistically significant after 2nd and 3rd month（P 〈0.05）. Undegradation Bio-oss were transported from replanted teeth root. Conclusion：A compound Bio-oss and rhBMP-2 appears to be useful for repairing the alveolar bone defects around implanted teeth.

关 键 词：BIO-OSS 骨形成蛋白 牙再植 骨结合 

分 类 号：R783.6[医药卫生—口腔医学]