氧代赖氨酸的抗肿瘤作用及其免疫学活性  

Antitumor and immunological activities of oxalysine

作　　者：王兴旺[1] 胥彬[1] 

机构地区：[1]中国科学院上海药物研究所

出　　处：《中华肿瘤杂志》1997年第2期115-117,共3页Chinese Journal of Oncology

基　　金：国家自然科学基金

摘　　要：研究氧代赖氨酸（ＯＸＬ）抗肿瘤作用及其免疫学活性。方法用ＭＴＴ法体外观察小鼠腹水肝癌－２２（Ｈ－２２）细胞的生长及其脾淋巴细胞增殖。结果ＯＸＬ（６．３～２００μｇ／ｍｌ）单用对Ｈ－２２细胞生长的抑制作用较小；但与５－氟脲嘧啶（５－Ｆｕ）合用，可协同抑制Ｈ－２２细胞生长，抑制率由单用ＯＸＬ（２５μｇ／ｍｌ）时的１３．８％或单用５－Ｆｕ（３．１μｇ／ｍｌ）时的１８．９％，提高到合用时的４３．１％。ＯＸＬ（６．３～１００μｇ／ｍｌ）对正常小鼠脾淋巴细胞增殖有抑制作用，但明显增强荷Ｈ－２２小鼠脾淋巴细胞增殖。结论ＯＸＬ对Ｈ－２２细胞生长有抑制作用。Objective To study the antitumor and immunological activities of oxalysine (OXL). Methods Growth of mouse ascites hepatoma 22 (H 22) cells and splenocyte proliferation were investigated in vitro by MTT colorimetric assay. Results Suppression of OXL (6.3～200 μg/ml) on H 22 cell growth was weaker when used singly. But a marked synergetic inhibition was observed when OXL was used in combination with 5 fluorouracil (5 Fu), with suppression percentage on hepatoma growth increased to 43.1% from 13.8% (25 μg/ml of OXL alone) or 18.9% (3.1 μg/ml of 5 Fu alone). OXL (6.3～100 μg/ml) in vitro produced an inhibitory action on splenocyte proliferation induced by concanvilian A (Con A, 5 μg/ml) in the normal mice, but was able to enhance obviously the proliferative response in mice bearing H 22. Conclusion OXL inhibits H 22 cell growth. The immunoregulatory action of OXL may contribute to its antihepatoma activity.

关 键 词：氧化赖氨酸 肝肿瘤 免疫活性 

分 类 号：R735.705[医药卫生—肿瘤]