球囊损伤犬颈动脉后IGF-1表达动态变化及血管再狭窄形成  

Dynamic alteration of the expression of IGF-1 in blood vessel endothelium after carotid artery stenosis caused by ballon angioplasty in canine model

作　　者：范晨[1] 刘一之[1] 周岱[2] 王中[2] 

机构地区：[1]苏州大学附属第一医院介入科 [2]苏州大学附属第一医院神经外科,江苏苏州215006

出　　处：《中国临床解剖学杂志》2007年第5期556-559,563,共5页Chinese Journal of Clinical Anatomy

基　　金：江苏省135工程资助基金项目(36RC2002036)

摘　　要：目的:研究球囊损伤后犬颈动脉内皮后胰岛素样生长因子-1(IGF-1)的表达及血管再狭窄形成过程。方法:采用ELISA法检测IGF-1在犬颈动脉球囊损伤后血管狭窄形成过程中的表达动态变化,同时采用免疫组化结合影像学测量方法观察犬颈动脉狭窄形成过程。结果:IGF-1的释放在球囊血管成形术后4～6h(平均值942.55±139.56ng/ml、1073.09±277.43ng/ml)启动,术后12h(平均值1468.35±322.08ng/ml)达到峰值,至术后72h(平均值1405.64±269.08ng/ml)维持高水平释放;犬颈动脉球囊损伤后4周时增殖细胞核抗原(PCNA)阳性细胞数(平均值61.60±6.19个/1000倍视野)达到峰值,4～8周期间α-肌动蛋白(α-actin)染色显示血管内皮增生性变化,直至术后8周左右血管狭窄率(62.76±9.57)%达到峰值。结论:犬颈动脉球囊损伤后4周内即发生血管增生性变化,至术后8周左右血管狭窄形成;颈动脉内皮损伤后早期即有IGF-1表达增强,可能为血管成形术后动脉狭窄发生的启动信息之一。Objoective：To study the expression of insulin-like growth factor-1 （IGF-1） in blood vessel endothelium after carotid artery stenosis caused by ballon angioplasty in canine model. Methods： The expression of IGF-1 after carotid artery stenosis was examined by ELISA, the process of stenosis was analyzed by immunohistochemistry and imageology. Results： The release of IGF-1 increased at 4 -6 hours after operation （average 942.55± 139.56 ng/ml, 1073.09±277.43 ng/ml）, and the peak value arrived at 12 hours after operation （average 1468.35±322.08 ng/ml）and maintained about 72 hours （average 1405.64±269.08 ng/ml）. The number of PCNA positive cells reached the maximum （61.60±6.19/Vf× 1000 ）at 4 weeks, and endotheliosis of carotid artery showed by α-actin immunohistochemistry appeared during 4 - 8 weeks, and angiostegnosis rate reached the peak after 8 weeks, Conclusions： Pathologic hypertrophic alteration of blood vessel occurs in 4 weeks after ballon angioplasty in canine model, and stenosis forms stably at 8 weeks. Enhancement of IGF-1 expression occurs early after carotid artery endothelium injury, which might be one of initiation information related to carotid artery restenosis.

关 键 词：颈动脉再狭窄 球囊血管成形术 介入 

分 类 号：R743.3[医药卫生—神经病学与精神病学]