CpG-ODN活化的外周血单个核细胞体外对肿瘤细胞杀伤效应的研究  

Study on antitumor effect of human peripheral blood mononuclear cells activated by CpG-ODN in vitro

作　　者：章保新[1] 范学工[1] 李宁[2] 应若素[1] 

机构地区：[1]中南大学湘雅医院感染病科,湖南长沙410008 [2]中南大学湘雅医院输血科,湖南长沙410008

出　　处：《中国现代医学杂志》2007年第18期2215-2217,2229,共4页China Journal of Modern Medicine

摘　　要：目的探讨CpGODN体外活化的外周血单个核细胞(PBMC)后对肿瘤细胞的杀伤效应。方法CpG-ODN体外刺激人外周血单个核细胞(PBMC),将活化的PBMC与肿瘤细胞按50∶1的比例共孵育72h后,以MTT和酶学检测活化的PBMC对肿瘤细胞的杀伤作用。结果CpG-ODN诱导活化的PBMC对肿瘤细胞的杀伤作用增强,而对正常肝细胞则无明显的杀伤作用。结论CpG-ODN可诱发机体免疫效应,提高对肿瘤细胞的杀伤作用。[Objective] To study the antitumor effect of human peripheral blood mononuclear cells（PBMC） activated by synthetic oligodeoxynucleotides（ODN） containing unmethylated CpG dinucleotides（CpG-ODN） in vitro. [Methods] CpG-ODN was used to stimulate PBMC. PBMC activated by CpG-ODN was co-cultured with QSG7701, HepG2, K562, A549 and 5-8F cells respectively for 72 h. MTT method and enzyme assays in the supernatant were uesd to detect PBMC-mediated antitumor activity. [Results] CpG-ODN enhanced antitumor activity of PBMCs, but it didn＇t up-regulate the PBMC killing activity to QSG7701 cells. [Onclusion] The study indicates that, as a novel immunoregulator, CpG-ODN can enhance antitumor activity by stimulating PBMC in vitro.

关 键 词：CPG寡脱氧核苷酸 外周血单个核细胞(PBMC) 免疫调节 

分 类 号：R730.51[医药卫生—肿瘤]