HBx小鼠肿瘤细胞株的建立及初步鉴定  

ESTABLISHMENT AND IDENTIFICATION OF AN IN VITRO MURINE CARCINOMA CELL LINE WITH HBX GENE EXPRESSION

作　　者：汤仁仙[1] 尤红娟[1] 徐龙涛 孙亚峰[3] 王慧[1] 裴冬生[3] 

机构地区：[1]徐州医学院病原生物学教研室,江苏徐州221002 [2]齐鲁动物保健品有限公司,山东济南250100 [3]徐州医学院生物化学与分子生物学研究中心,江苏徐州221002

出　　处：《山东农业大学学报（自然科学版）》2007年第3期342-344,共3页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：目的建立稳定、高效的HBxAg体外表达细胞株,以便进一步研究HBX基因和HBxAg的致癌作用及机理。方法用RT-PCR技术扩增出了HBX的cDNA,并克隆到真核表达载体pcDNA3.1中,通过脂质体介导将pcD-NA3.1-HBX转染到小鼠肿瘤细胞株(EL4)中。结果RT-PCR及蛋白质印迹的实验结果表明,HBX基因在小鼠肿瘤细胞EL4细胞中得到了表达。结论本研究成功构建了表达HBx的小鼠肿瘤细胞株,为进一步研究HBX基因和HBx的致癌作用及机制奠定了实验基础。To establish in vitro cell line with stable and effective HBxAg expression was established in order study to carcinogenesis mechanism of HBX gene and HBxAg. A recombinant plasmid （pcDNA3. 1 - HBX） containing the full length of HBX gene was transfected into EIA cells. The expression of HBx in the cells was analyzed by Western blot using anti - HBx antibodies. Cell clones were obtained after being transfected with pcDNA3.1 - HBX plasmid. Stable expression of HBxAg was detected by Western blot and RT - PCR. AHBX gene expression cell line was constructed in this study. The property of this system ensures stable and high level expression of HBx. It is a useful tool for functional studies of HBX gene.

关 键 词：HBX 细胞株 基因表达 

分 类 号：Q813.2[生物学—生物工程]