肺缺血再灌注损伤时一氧化碳的变化及葛根素对其影响  

Variations of carbon monoxide system in lung ischemia-reperfusion injury and effects of puerarin

作　　者：邱晓晓 王万铁[1] 徐正[1] 戴雍月[1] 陈锡文[2] 方周溪[3] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035 [2]温州医学院实验动物中心,浙江温州325035 [3]温州医学院电镜室,浙江温州325035

出　　处：《温州医学院学报》2007年第5期469-471,474,共4页Journal of Wenzhou Medical College

基　　金：温州市科技计划重点项目(Y2005A080)

摘　　要：目的:观察兔肺缺血再灌注损伤时一氧化碳的变化及葛根素对其的影响。方法:30只健康日本大耳白兔随机分成假手术对照组(C组)、肺缺血再灌注组(IR组)和葛根素组(Pur组)。复制兔单侧肺缺血再灌注损伤模型,各组在缺血前、缺血1h、再灌注1h、3h和5h分别抽血,检测一氧化碳血红蛋白(COHb)浓度、环磷酸鸟苷(cGMP)含量。实验结束时取肺组织检测cGMP含量,电镜观察肺组织超微结构的改变。结果:血浆COHb浓度、cGMP含量和肺组织cGMP含量IR组、Pur组明显高于C组,以Pur组为著(P<0.01)。电镜显示IR组有明显的肺超微结构损伤,而Pur组损伤较轻。结论:葛根素可通过提高内源性CO水平,对缺血再灌注损伤肺组织发挥保护作用。Objective：To study variations of carbon monoxide in lung ischemia-reperfusion injury and effects of puerarin. Methods：The unilateral lung ischemia-reperfusion model was replicated in vivo. Rabbits were randomly divided into three groups （n=10 in each）, control （C） group, ischemia-reperfusion （IR） group and puerarin （Pur） group. The blood specimens gathered before ischemia, ischemia 1 h, reperfusion 1 h, 3 h and 5 h were tested for the content of carboxyhemoglobin （COHb）, cyclic guanosine monophosphate（cGMP）. The lung tissues sampled at 5 hours after reperfusion were assayed for the content of cGMP, the ultrastructural changes were observed under electron microscope. Results：The plasma content of COHb, the content of cGMP of plasma and lung tissue in IR and Pur group were much higher than that in C group, and the index in Pur group increased more significantly（P 〈 0.01）. Severe ultrastructural morphological damage in IR group was observed under eletron microscope, but lightly injury was found in Pur group. Conclusion：Puerarin provides significant protective effects on lung during ischemia-reperfusion injury through increasing the plasma content of COHb.

关 键 词：肺 缺血再灌注损伤 一氧化碳 环磷酸鸟苷 葛根素 

分 类 号：R363[医药卫生—病理学]