高功率微波辐射对大鼠脑HIF-1α表达影响  

Expression and significance of hypoxia inducible factor-1 alpha in rat brain after HPM exposure

作　　者：王旭[1] 胡向军[1] 彭瑞云[1] 王水明[1] 高亚兵[1] 王丽峰[1] 吴振宇[1] 魏丽[1] 马俊杰[1] 胡文华[1] 苏镇涛[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所实验病理研究室,北京100850

出　　处：《中国公共卫生》2007年第10期1161-1163,共3页Chinese Journal of Public Health

基　　金：全军"十一五"医药卫生专项项目(06Z065)

摘　　要：目的探讨高功率微波(high power microwave,HPM)辐射后大鼠不同脑区低氧诱导因子1α(hypoxia in-ducible factor-1 alpha,HIF-1α)的改变及其意义。方法60只Wistar雄性大鼠于0,30和100mW/cm2HPM辐射后6h,1,3和7d时活杀取大脑皮层和海马,采用苏木素-伊红染色观察形态改变,采用免疫组织化学、原位杂交和图像分析等技术检测大鼠不同脑区HIF-1α蛋白和mRNA表达改变。结果对照组大鼠皮层和海马毛细血管形态规则,未见毛细血管间隙增宽;30和100m Wcm2组大鼠皮层和海马脑辐射后6h～1d毛细血管周隙增宽,3d达高峰,7d后恢复;对照组HIF-1α蛋白于皮层和海马神经细胞呈弱阳性,30和100m Wcm2组皮层和海马神经元和胶质细胞核HIF-1α于辐射后6h～1d呈阳性,其后呈弱阳性;HIF-1α mRNA改变规律与其蛋白变化相同。结论脑内神经元的损伤和修复过程中HIF-1α表达增加,参与了毛细血管通透性升高的过程。Objective To investigate the expression and significance of HIF-1α in the brain of rats after HPM exposure. Methods 60 Wistar rats were sacrificed after the exposure of 0, 30 and 100 mW/cm^2 H＇PM at 6h, ld, 3d and 7d to obtain the brains. HE stain was used to investigate the morphous of blood capillary; Immunohistochemistry, in situ hybridization and quantitative analysis methods were used to investigate the HIF-1α protein and mRNA expression. Results The morphous of capillary in sham exposure groups were regular and no widen gap were found; more widen gaps were found in 30 and 100 mW/cm^2 exposure group. The expression of HIF-1α protein was weakly positive in sham groups, which increased in 30 and 100 mW/cm^2 groups during 6 h to 1 d, and then decreased. Conclusion The increased expression of HIF-1α participate in the injury and reparative process in the brain.

关 键 词：HPM 脑 毛细血管 通透性 缺氧诱导因子 

分 类 号：R142.4[医药卫生—公共卫生与预防医学]