维生素D_3对大鼠卵巢切碎组织性激素分泌影响  

Effects of vitamin D_3 on sex hormones by chipped ovaries of rats

作　　者：朱建林[1] 张文昌[1] 李宏[1] 黄意坚[1] 

机构地区：[1]福建医科大学公共卫生学院职业与环境卫生学系,福州350004

出　　处：《中国公共卫生》2007年第10期1163-1164,共2页Chinese Journal of Public Health

摘　　要：目的研究维生素D3(VD3)在动情周期不同阶段对大鼠卵巢切碎组织分泌雌二醇(E2),孕酮(P4)的影响。方法采用正交表L16(45)设计实验,研究VD3(10-4,10-7,10-10mol/L在动情周期的4个阶段(动情前期、动情期、动情后期、动情间期)对卵巢切碎组织分泌E2和P4的影响。结果VD3可影响卵巢切碎组织体外分泌E2(P<0.001),但不会影响分泌P4(P>0.05),卵巢切碎组织体外分泌E2和P4与所处的动情周期有关(P<0.001,P<0.05)。结论VD3对卵巢类固醇激素生成和分泌有直接影响。Objective To study the effects of vitamin D3 on steroidogenesis and secretion in vitro by chipped ovaries of rats. Methods Using orthogonal experimental design（L16（4^5） ）, two factorial experiments were designed to explore the influence of four dosage levels of vitamin VD3（10^-4,10^-7,10^-10mol/L and four different stages（proestrous, estmus, postestrous, dioestrous）of estrous cycle on steroidogenesis in the ovary. Results In chipped ovary culture, effects of vitmin D3 on estradiol secretion were significant （P 〈 0.001）, and effects of vitamin D3 on progesterone secretion were not significant （P 〉 0.05）. Effects of estrous cycle on estradlol and progesterone secretion were significant（P 〈 0. 001, P 〈 0.05）. Conclusion There is direct effects of vitamin D3 on sex hormones secretion in intro by ovaries, and vitamin D3 may be one of the environmental endocrine disruptors（EEDs）.

关 键 词：维生素D3(VD3) 卵巢切碎组织培养 性激素 

分 类 号：R742.1[医药卫生—神经病学与精神病学]