CXJZ11-01菌株分泌中性β-甘露聚糖酶的研究  

Study on Neutral β-Mannannase Secreted by CXJZ11-01 Strain

作　　者：成莉凤[1] 刘正初[1] 张运雄[1] 段盛文[1] 冯湘沅[1] 郑科[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410125

出　　处：《中国麻业科学》2007年第5期264-269,共6页Plant Fiber Sciences in China

基　　金：国家"863"计划课题2004AA214110

摘　　要：对现有菌种资源进行提纯复壮和筛选,采用透明圈法和发酵液酶活力比较法,获得高产中性β-甘露聚糖酶菌株CXJZ11-01。进而采用单因子和多因子相结合的统计方法以及DNS法,对该菌株β-甘露聚糖酶活力检测体系及其产酶规律进行系统研究。结果表明:该中性β-甘露聚糖酶最适pH值为6.0,最适温度为65℃;菌株CXJZ11-01在适宜条件下培养9小时,发酵液粗酶活力高达3050U/ml。By clear halos and the crude β-Mannannase activity in fermented liquid, strain CXJZ11-01 was selected from several strains which could secrete β-Mannannase. Then its enzyme pro- duction law and main factors affecting β-Mannannase activity were systematically studied. The optimal reaction pH and temperature of the crude β-Mannannase were 6.0 and 65℃ respectively. When strain CXJZ11-01 had been cultured for 9 hours in optimum condition, its β-Mannannase activity in fermented liquid reached to peak value, 3050U/mL.

关 键 词：魔芋精粉 中性β-甘露聚糖酶 透明圈 产酶规律 

分 类 号：S816.7[农业科学—饲料科学]