ABCG2、VEGF、IGFBP-2、EGFR在胶质瘤组织芯片中表达与预后关系的分析  

The analysis on the relationship between expressions of ABCG2,VEGF,IGFBP-2 and EGFR in glioma tissue microarray and prognosis

作　　者：肖宏[1] 黄强[1] 董军[1] 翟德忠[1] 宁小明[2] 王爱东[1] 兰青[1] 

机构地区：[1]苏州大学附属第二医院神经外科暨脑肿瘤研究室,苏州215004 [2]哈尔滨医科大学附属第三医院病理科,哈尔滨150040

出　　处：《中华神经医学杂志》2007年第9期878-882,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(30371457;30672164)

摘　　要：目的探讨干细胞标志蛋白(ABCG2)、血管内皮生长因子(VEGF)、胰岛素样生长因子结合蛋白(IGFBP-2)和内皮细胞生长因子受体(EGFR)在胶质瘤中的表达及与预后的关系。方法构建布有各级别人脑胶质瘤标本的组织芯片,进行ABCG2、VEGF、IGFBP-2和EGFR的EnVision法、SP法免疫组化染色,分析其在胶质瘤中的表达率,并根据随访资料对可能影响预后的因素进行统计学分析。结果ABCG2、VEGF、IGFBP-2和EGFR的阳性表达率在胶质瘤恶性度高低组之间存在显著差异(P<0.05)。Logistic回归单因素分析显示术前Karnofsky评分(KPS)、手术切除范围、病理级别、肿瘤大小、术后放疗以及ABCG2、VEGF基因的表达对3年生存率有意义。多因素分析结果显示术前KPS评分、手术切除范围、病理级别、术后放疗、ABCG2和VEGF基因的表达是影响病人预后的因素。结论ABCG2、VEGF、IGFBP-2和EGFR的阳性表达与胶质瘤恶性程度相关。术前KPS评分、手术切除范围、病理级别、术后放疗、ABCG2和VEGF基因的表达可作为考察胶质瘤患者预后情况的重要指标。Objective To examine the expressions of ATP-binding cassette subfamily G member 2 （ABCG2）, vascular endothelial growth factor （VEGF）, insulin-like growth factor binding protein-2 （IGFBP-2） and epidermal growth factor receptor （EGFR） in gliomas, and study the correlation between the expressions and prognosis of gliomas. Methods The tissue microarray （TMA） containing 90 specimens that included all pathological grades of human brain glioma tissues was constructed and the EnVision detection system and SP immunohistochemical staining technique was used to detect the expression rates of ABCG2, VEGF, IGFBP-2 and EGFR in gliomas; the factors which might affect the prognosis of gliomas were statistically analyzed according to the data of the follow-up study. Results The positive expression rates of ABCG2, VEGF, IGFBP-2 and EGFR of low-grade gliomas were significantly different from that of high-grade gliomas, which were graded by the degree of malignancy （P〈0.05）. Univariate logistic regression analysis showed that the effects of clinical factors including the preoperative Karnofsky performance status （KPS） score, resection extent, pathological grade and size of the tumor, postoperative radiotherapy, and the expressions of ABCG2, VEGF on the 3-year survival rate of the patients had statistical significance. Multivariate logistic regression analysis showed that the preoperative KPS score, resection extent, pathological grade, postoperative radiotherapy and the expressions of ABCG2 and VEGF were the factors correlating to the prognosis of patients. Conclusion The positive expressions of ABCG2, VEGF, IGFBP-2 and EGFR are correlated with the degree of malignancy of gliomas. The preoperative KPS score, resection extent, pathological grade, postoperative radiotherapy and the expressions of ABCG2 and VEGF can be regarded as important prognostic indicators for patients with gliomas.

关 键 词：神经胶质瘤 干细胞标志蛋白 血管内皮生长因子 胰岛素样生长因子结合蛋白 内皮细胞生长因子受体 组织芯片 预后因素 

分 类 号：R739.4[医药卫生—肿瘤]