硼中子俘获疗法治疗小鼠颅内G422胶质细胞瘤  

Boron neutron capture therapy for the treatment of G422 glioma

作　　者：王鹏[1] 章翔[1] 江新标[1] 程光[1] 姬西团[1] 赵英[1] 肖燕[1] 王剑博[1] 刘红[1] 

机构地区：[1]第四军医大学西京医院全军神经外科研究所,西安710032

出　　处：《中华神经医学杂志》2007年第9期892-895,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨硼中子俘获疗法(BNCT)治疗G422胶质细胞瘤的效果。方法建立小鼠颅内G422胶质细胞瘤模型,以ICP-AES法测定荷瘤小鼠不同组织中的^(10)B浓度将荷瘤小鼠随机分为未照射组(0 Gy)、γ射线对照组(5、10 Gy)、反应堆组(5、10Gy)、BNCT组(5、10Gy)采用生存时间的中位数、平均生存时间、生存时间延长率作为评价指标、观察各组的治疗效果。结果荷瘤小鼠腹腔注射对二羟苯丙氨酸硼溶液后1.5 h,瘤组织内的^(10)B浓度达到峰值[(43.78±3.02)μg/g]。BNCT 5、10 Gy照射后,移植G422胶质细胞瘤小鼠的生存时间延长率分别为235%(233%)、329%(342%)。BNCT 5 Gy组的生存率与未照射组、γ射线5 Gy组和反应堆5 Gy组相比差异有统计学意义(P<0.05)。BNCT 10 Gy组的生存率与未照射组、γ射线5 Gy组、γ射线10 Gy组、反应堆5 Gy组、反应堆10 Gy组、BNCT 5 Gy组相比差异有统计学意义(P<0.05)。结论BNCT可以显著提高荷G422胶质细胞瘤小鼠的生存率,并具有剂量依赖性的特点:同时BNCT具有较高的相对生物学效应,优于同剂量γ射线的治疗效果。Objective To explore the effect of boron neutron capture therapy（BNCT）on G422 glioma. Methods Intracranial G422 glioma models were established through the injection of G422 cells into the mice brains. The 10^B concentrations for different tissues of tumor-bearing mice were determined by inductively coupled plasma atomic emission spectroscopy （ICP-AES）. The tumor-bearing mice were randomly allocated into seven groups： unirradiated group （0 Gy）, γ-ray control groups （5, 10 Gy）, reactor groups （5, 10 Gy） and BNCT groups （5, 10 Gy）. The median survival time （MeST） and mean survival time （MST） were regarded as the evaluation indicators of therapeutic effects; the Kaplan-Meier survival curve was plotted to reflect the surviving process of each group; the percentage for increased life span （ILS, %） was calculated by the formula put forward by Barth. Results The 10^B concentration in tumor tissues reached the peak value of （43.78±3.02） μg/g at 1.5 h after intraperitoneal injection of boronophenylalanine （BPA） in the tumor-bearing mice. The percentages for increased life span of the mice with transplanted G422 glioma were respectively 235% （233%） and 329% （342%） in the BNCT groups （5, 10 Gy）. The survival rate of the 5-Gy BNCT group differed from that of the unirradiated group, 5-Gy γ-ray group and 5-Gy reactor group significantly （P〈0.05）. And, the survival rate of 10-Gy BNCT group differed from that of the unirradiated group, 5-Gyγ-ray group, 10-Gyγ-ray group, 5-Gy reactor group, 10-Gy reactor group and 5-Gy BNCT group significantly （P〈0.05）. Conclusion BNCT is an efficient therapy, which can improve the survival rate of mice bearing G422 glioma significantly in a dose-dependent manner. Compared with γ-ray irradiation, a higher relative biological effectiveness （RBE） can be achieved in vivo by BNCT.

关 键 词：硼中子俘获疗法 神经胶质瘤 动物模型 

分 类 号：R739.4[医药卫生—肿瘤]