平阳霉素乳剂对增生期毛细血管瘤细胞微环境的影响  

Pingyangmycin Emulsion Influencing on Microenvironment of Infantile Proliferating Capillary Hemangiomas

作　　者：徐伟立[1] 牛爱国[1] 李索林[1] 时保军[1] 

机构地区：[1]河北医科大学第二医院小儿外科,石家庄050000

出　　处：《肿瘤防治研究》2007年第9期690-692,738,共4页Cancer Research on Prevention and Treatment

基　　金：河北省卫生厅科研基金资助项目(2002045)

摘　　要：目的探讨平阳霉素(pingyangmycin,PYM)乳剂对增生期毛细血管瘤细胞微环境的影响,为血管瘤治疗提供新思路。方法选取婴儿体表增生期毛细血管瘤30例,随机分为两组,将PYM制成乳剂,涂于用药组瘤体表面,1次/8小时;对照组则只涂以基质。用药7天后,手术切除瘤体,检测两组瘤体组织中VEGF、细胞外基质及细胞凋亡指数,分别进行统计学处理。另各取两组2例电镜观察细胞超微结构变化。结果两组间VEGF表达(P<0.05)和细胞外基质表达均有显著性差异(P<0.01);细胞凋亡指数比较有非常显著性差异(P<0.01)。结论外用PYM乳剂可通过降低增生期毛细血管瘤组织中VEGF表达、抑制细胞外基质合成与再生和诱导细胞凋亡而促进其消退。Objective To explore pingyang mycin（PYM） emulsion influencing on the microenvironment of infantile proliferating capillary hemangiomas so as to find the optimal method for hemangiomas＇ treatment. Methods Thirty samples of infantile proliferating hemangiomas were divided into control group and experimental group. PYM was made into emulsion and smeared on the surfaces of the leision in experimental group with 3 times every day as well as only matrix in control group. The specimens were cut off on day 7, then made into pathological slices and electron microscope slices in order to observe the cells ultrastructure changes. Furthermore, VEGF, extracellular matrix（P〈0. 01） and apoptotic index in hemangiomas were detected respectively. Results VEGF （P〈0. 05）, extracellular matrix（P〈0. 01 ） and apoptotic index （P〈0. 01） of two groups had all significant difference. Conclusion PYM emulsion can effectively accelerate regression of proliferating capillary hemangiomas whose mechanism is related to the reduction of VEGF expression and prevention of synthesis and regeneration of extracellular matrix and induction of cell apoptosis.

关 键 词：平阳霉素 血管瘤 VEGF 细胞外基质 细胞凋亡 

分 类 号：R730.53[医药卫生—肿瘤] R732.23[医药卫生—临床医学]