青刺果促腋芽分枝快繁及生根诱导  

Research on the Enhanced Axillary Branching and Rooting Induction of Prinsepia utilis Royle

作　　者：曾妮[1] 李化[1] 唐琳[1] 肖科[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,四川成都610064

出　　处：《安徽农业科学》2007年第27期8554-8555,共2页Journal of Anhui Agricultural Sciences

基　　金：国家"十五"科技攻关课题

摘　　要：研究青刺果的快繁技术。通过腋芽丛生方式对青刺果组织培养中种子的灭菌,腋芽丛生,壮苗培养,生根诱导等进行了研究,得出适合青刺果离体培养的培养体系。培养基MS＋6-BA 2.0 mg/L＋NAA 0.1 mg/L的激素搭配较适合青刺果的腋芽诱导,平均每个外植体可得到6-10个丛生芽。培养基MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L明显促进茎的增粗和叶片的扩展。适宜的生根培养基为改良的1/2MS＋IBA 1.0 mg/L,生根率可达85%。生根苗经炼苗处理后移栽到蛭石∶营养土（1∶1）的花盆中,成活率达80%以上。青刺果带腋芽的茎段丛生增殖效果好。在培养基中添加不同种浓度的各种生长素对再生芽进行培养之后生根效果较好,移栽成活率也较高。Explants from off-spring were used to study axillary bud proliferation,regenerated shoot and rooting in vitro.The results showed that seeds were firstly sterilized in 70% ethanol for 30s and in 0.1% HgCl2 for 8 minutes,file episperm was pared and then it was cultured on MS base medium.The contamination rate was low and the survival rate was 80%. Multiple shoot induction was obtained in MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.1 mg/L, with 6-10 shoots, respectively. Regenerated shoots were strengthened in MS ＋ 6-BA 0.5 ng/L ＋ NAA 0.1 mg/L, then rooted in reforming 1/2MS ＋ IBA 1.0 mg/L, and the ratio of rhizogenesis was 85%. After one-week trained,rooted-shoots were transplant into flowerpots with vermiculite and nourish soil （1：1 ）. The survival rate could achieve 80%.

关 键 词：青刺果 组织培养 腋芽丛生 快速繁殖 

分 类 号：Q943.1[生物学—植物学]