粮油作物中微量铜测定的光度法研究  

Research on the Determination of Trace Copper in Crops by Using Sepctrophotometry Method

作　　者：冯爱青[1] 胡秋娈[1] 张玉松[2] 

机构地区：[1]洛阳师范学院生命科学系,河南洛阳471022 [2]洛阳师范学院化学化工学院,河南洛阳471022

出　　处：《洛阳师范学院学报》2007年第5期78-80,共3页Journal of Luoyang Normal University

摘　　要：在pH2.5～5.0条件下,Cu^(2+)与红色的氯磺酚S(CS)作用生成蓝色配合物,其最大吸收波长为650nm,比CS本身红移86nm。铜含量在0～20μg/10mL范围内符合Beer定律,表观摩尔吸光系数为6.87×10~4L·mol^(-1)·cm^(-1)。研究了CS与Cu^(2+)的光谱变化、反应条件、表面活性剂及共存物质的影响。十二烷基苯磺酸钠有增敏作用。方法操作简便,快速,灵敏度高,用于多种粮食,油料作物中铜(Ⅱ)含量的测定,结果满意。The organic dyes ChlorosulfophenolS （CS）-Spectrophotometry was applied to determine the microcopper in crops. The change of spectrum and the reaction conditions of Cu（ Ⅱ ） with CS have been studied. The result showed that Cu（ Ⅱ ） reacts with CS and generates the blue complex in Britton-Robinson buffer medium at pH = 2.5 - 5.0, which presents maximum absorption at 650nm with 86nm of red shift compared to CS itself. Beer＇s law is followed the rang of 0 -20μg/10mL, and the apparent mole absorptivity is 2.0 × 10^4L · mo1^-1 · cm^-1. The proposed method was used for the determination of copper crops.

关 键 词：氯磺酚S 分光光度法 微量铜 

分 类 号：O657.3[理学—分析化学]